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1bqk

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(New page: 200px<br /> <applet load="1bqk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bqk, resolution 1.35&Aring;" /> '''OXIDIZED PSEUDOAZUR...)
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[[Image:1bqk.gif|left|200px]]<br />
 
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<applet load="1bqk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bqk, resolution 1.35&Aring;" />
 
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'''OXIDIZED PSEUDOAZURIN'''<br />
 
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==Overview==
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==OXIDIZED PSEUDOAZURIN==
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The crystal structures of oxidized and reduced pseudoazurins from a, denitrifying bacterium, Achromobacter cycloclastes IAM1013, have been, determined at 1.35- and 1.6-A resolutions, respectively. The copper site, in the oxidized state exhibits a distorted tetrahedral structure like, those of other pseudoazurins. However, not only a small change of the, copper geometry, but concerted peptide bond flips are identified. The, imidazole ring of remote His6 has a hydrogen bonding distance of 2.73 A, between N-delta1(His6) and O-gamma1(Thr36) in the oxidized protein. When, the protein is reduced at pH 6.0, the imidazole ring rotates by 30.3, degrees and moves 1.00 A away from the position of the oxidized state. A, new hydrogen bond between N-epsilon2(His6) and O-epsilon1(Glu4) is formed, with a ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10364229 (full description)]]
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<StructureSection load='1bqk' size='340' side='right'caption='[[1bqk]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1bqk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BQK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bqk OCA], [https://pdbe.org/1bqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bqk RCSB], [https://www.ebi.ac.uk/pdbsum/1bqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bqk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AZUP_ACHCY AZUP_ACHCY] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bq/1bqk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bqk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structures of oxidized and reduced pseudoazurins from a denitrifying bacterium, Achromobacter cycloclastes IAM1013, have been determined at 1.35- and 1.6-A resolutions, respectively. The copper site in the oxidized state exhibits a distorted tetrahedral structure like those of other pseudoazurins. However, not only a small change of the copper geometry, but concerted peptide bond flips are identified. The imidazole ring of remote His6 has a hydrogen bonding distance of 2.73 A between N-delta1(His6) and O-gamma1(Thr36) in the oxidized protein. When the protein is reduced at pH 6.0, the imidazole ring rotates by 30.3 degrees and moves 1.00 A away from the position of the oxidized state. A new hydrogen bond between N-epsilon2(His6) and O-epsilon1(Glu4) is formed with a distance of 3.03 A, while the hydrogen bond between N-delta1(His6)-O-gamma1(Thr36) is maintained with an interatomic distance of 2.81 A. A concomitant peptide bond flip of main chain between Ile34 and Thr36 occurs.
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==About this Structure==
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Crystal structure determinations of oxidized and reduced pseudoazurins from Achromobacter cycloclastes. Concerted movement of copper site in redox forms with the rearrangement of hydrogen bond at a remote histidine.,Inoue T, Nishio N, Suzuki S, Kataoka K, Kohzuma T, Kai Y J Biol Chem. 1999 Jun 18;274(25):17845-52. PMID:10364229<ref>PMID:10364229</ref>
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1BQK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Achromobacter_cycloclastes Achromobacter cycloclastes]] with CU as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BQK OCA]].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure determinations of oxidized and reduced pseudoazurins from Achromobacter cycloclastes. Concerted movement of copper site in redox forms with the rearrangement of hydrogen bond at a remote histidine., Inoue T, Nishio N, Suzuki S, Kataoka K, Kohzuma T, Kai Y, J Biol Chem. 1999 Jun 18;274(25):17845-52. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10364229 10364229]
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</div>
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[[Category: Achromobacter cycloclastes]]
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<div class="pdbe-citations 1bqk" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Hamanaka, S.]]
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[[Category: Harada, S.]]
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[[Category: Inoue, T.]]
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[[Category: Iwasaki, H.]]
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[[Category: Kai, Y.]]
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[[Category: Kohzuma, T.]]
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[[Category: Nishio, N.]]
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[[Category: Shidara, S.]]
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[[Category: Shimomura, T.]]
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[[Category: Suzuki, S.]]
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[[Category: CU]]
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[[Category: cuproprotein]]
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[[Category: electron transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:59:10 2007''
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==See Also==
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*[[Pseudoazurin|Pseudoazurin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Achromobacter cycloclastes]]
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[[Category: Large Structures]]
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[[Category: Hamanaka S]]
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[[Category: Harada S]]
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[[Category: Inoue T]]
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[[Category: Iwasaki H]]
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[[Category: Kai Y]]
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[[Category: Kohzuma T]]
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[[Category: Nishio N]]
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[[Category: Shidara S]]
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[[Category: Shimomura T]]
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[[Category: Suzuki S]]

Current revision

OXIDIZED PSEUDOAZURIN

PDB ID 1bqk

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