This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1hrs

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1hrs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hrs, resolution 2.6&Aring;" /> '''A CRYSTALLOGRAPHIC ST...)
Current revision (07:29, 7 February 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1hrs.gif|left|200px]]<br /><applet load="1hrs" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1hrs, resolution 2.6&Aring;" />
 
-
'''A CRYSTALLOGRAPHIC STUDY OF HAEM BINDING TO FERRITIN'''<br />
 
-
==Overview==
+
==A CRYSTALLOGRAPHIC STUDY OF HAEM BINDING TO FERRITIN==
-
Ferritin, the iron-storage protein, binds porphyrins, metalloporphyrins, and the fluorescent dyes ANS (8-anilino-1-naphthalenesulfonic acid) and, TNS (2-p-toluidinyl-6-naphthalenesulfonic acid), similarly to, apo-myoglobin. Octahedral crystals of horse-spleen apo-ferritin (HSF; 174, amino acids) complexes prepared by the addition of haem, hematoporphyrin, or Sn-protoporphyrin IX to a solution of apo-ferritin crystallize in space, group F432 with cell parameter a = 184.0 A. X-ray crystallographic, analysis of single crystals prepared from a mixture containing haem or, Sn-protoporphyrin IX shows that the haem-binding sites in these crystals, are occupied by protoporphyrin IX, which is free of metal, rather than by, the original metalloporphyrin. The present paper describes the structure, of horse-spleen apo-ferritin cocrystallized with Sn-protoporphyrin IX. The, 6797 reflections up to 2.6 A resolution used in the refinement were, obtained from a data set recorded on a Nicolet/Xentronics area detector, with Cu Kalpha radiation from a Rigaku RU 200 rotating anode. The final, structure comprises 1613 non-H atoms, two Cd atoms and 170 solvent, molecules. Four residues are described as disordered. The root-mean-square, deviations from ideal bond lengths and angles are 0.013 A and 2.88, degrees, respectively. Protoporphyrins are observed in special positions, on the twofold axes of the ferritin molecule with a stoichiometry of 0.4, per subunit.
+
<StructureSection load='1hrs' size='340' side='right'caption='[[1hrs]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1hrs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Equus_caballus Equus caballus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HRS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HRS FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=PP9:PROTOPORPHYRIN+IX'>PP9</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hrs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hrs OCA], [https://pdbe.org/1hrs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hrs RCSB], [https://www.ebi.ac.uk/pdbsum/1hrs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hrs ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/FRIL_HORSE FRIL_HORSE] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hr/1hrs_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hrs ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1HRS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Equus_caballus Equus caballus] with CD and PP9 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HRS OCA].
+
*[[Ferritin 3D structures|Ferritin 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
A crystallographic study of haem binding to ferritin., Precigoux G, Yariv J, Gallois B, Courseille C, d'Estaintot BL, Acta Crystallogr D Biol Crystallogr. 1994 Sep 1;50(Pt 5):739-43. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299370 15299370]
+
[[Category: Equus caballus]]
[[Category: Equus caballus]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Courseille, C.]]
+
[[Category: Courseille C]]
-
[[Category: Dautant, A.]]
+
[[Category: Dautant A]]
-
[[Category: Estaintot, B.Langlois.D.]]
+
[[Category: Gallois B]]
-
[[Category: Gallois, B.]]
+
[[Category: Langlois D'Estaintot B]]
-
[[Category: Precigoux, G.]]
+
[[Category: Precigoux G]]
-
[[Category: Yariv, J.]]
+
[[Category: Yariv J]]
-
[[Category: CD]]
+
-
[[Category: PP9]]
+
-
[[Category: iron storage]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:47:25 2007''
+

Current revision

A CRYSTALLOGRAPHIC STUDY OF HAEM BINDING TO FERRITIN

PDB ID 1hrs

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1hrs"
Personal tools