1kix

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{{Seed}}
 
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[[Image:1kix.png|left|200px]]
 
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==Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA==
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The line below this paragraph, containing "STRUCTURE_1kix", creates the "Structure Box" on the page.
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<StructureSection load='1kix' size='340' side='right'caption='[[1kix]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1kix]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1KIX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1kix| PDB=1kix | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1kix FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1kix OCA], [https://pdbe.org/1kix PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1kix RCSB], [https://www.ebi.ac.uk/pdbsum/1kix PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1kix ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TEBA_STENO TEBA_STENO] May function as protective capping of the single-stranded telomeric overhang. May also participate in telomere length regulation during DNA replication. Binds specifically to the T4G4-containing extension on the 3'strand and protects this region of the telomere from nuclease digestion and chemical modification.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ki/1kix_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1kix ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Telomeres are the specialized protein--DNA complexes that cap and protect the ends of linear eukaryotic chromosomes. The extreme 3' end of the telomeric DNA in Oxytricha nova is bound by a two-subunit sequence-specific and 3' end-specific protein called the telomere end-binding protein (OnTEBP). Here we describe the crystal structure of the alpha-subunit of OnTEBP in complex with T4G4 single-stranded telomeric DNA. This structure shows an (alpha--ssDNA)2 homodimer with a large approximately 7,000 A2 protein--protein interface in which the domains of alpha are rearranged extensively from their positions in the structure of an alpha--beta--ssDNA ternary complex. The (alpha--ssDNA)2 complex can bind two telomeres on opposite sides of the dimer and, thus, acts as a protein mediator of telomere--telomere associations. The structures of the (alpha--ssDNA)2 dimer presented here and the previously described alpha--beta--ssDNA complex demonstrate that OnTEBP forms multiple telomeric complexes that potentially mediate the assembly and disassembly of higher order telomeric structures.
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===Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA===
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Dimeric structure of the Oxytricha nova telomere end-binding protein alpha-subunit bound to ssDNA.,Peersen OB, Ruggles JA, Schultz SC Nat Struct Biol. 2002 Mar;9(3):182-7. PMID:11836536<ref>PMID:11836536</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1kix" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_11836536}}, adds the Publication Abstract to the page
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*[[End-binding protein|End-binding protein]]
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(as it appears on PubMed at http://www.pubmed.gov), where 11836536 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11836536}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1KIX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIX OCA].
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==Reference==
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Dimeric structure of the Oxytricha nova telomere end-binding protein alpha-subunit bound to ssDNA., Peersen OB, Ruggles JA, Schultz SC, Nat Struct Biol. 2002 Mar;9(3):182-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11836536 11836536]
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[[Category: Single protein]]
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[[Category: Sterkiella nova]]
[[Category: Sterkiella nova]]
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[[Category: Peersen, O B.]]
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[[Category: Peersen OB]]
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[[Category: Ruggles, J A.]]
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[[Category: Ruggles JA]]
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[[Category: Schultz, S C.]]
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[[Category: Schultz SC]]
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[[Category: Dna-protein interaction]]
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[[Category: Single stranded dna]]
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[[Category: Ssdna]]
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[[Category: Telomere binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 10:24:43 2008''
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Current revision

Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA

PDB ID 1kix

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