1i18

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(New page: 200px<br /><applet load="1i18" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i18" /> '''SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN ...)
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[[Image:1i18.jpg|left|200px]]<br /><applet load="1i18" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i18" />
 
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'''SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF RIBOFLAVIN SYNTHASE FROM E. COLI'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF RIBOFLAVIN SYNTHASE FROM E. COLI==
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The structure of the amino-terminal domain of Escherichia coli riboflavin, synthase (RiSy) has been determined by NMR spectroscopy with riboflavin as, a bound ligand. RiSy is functional as a 75 kDa homotrimer, each subunit of, which consists of two domains which share very similar sequences and, structures. The N-terminal domain (RiSy-N; 97 residues) forms a 20 kDa, homodimer in solution which binds riboflavin with high affinity. The, structure features a six-stranded antiparallel beta-barrel with a, Greek-key fold, both ends of which are closed by an alpha-helix. One, riboflavin molecule is bound per monomer in a site at one end of the, barrel which is comprised of elements of both monomers. The structure and, ligand binding are similar to that of the FAD binding domains of, ferrodoxin reductase family proteins. The structure provides insights into, the structure of the whole enzyme, the organisation of the functional, trimer and the mechanism of riboflavin synthesis. C48 from the N-terminal, domain is identified as the free cysteine implicated in a nucleophilic, role in the synthesis mechanism, while H102 from the C-terminal domains is, also likely to play a key role. Both are invariant in all known riboflavin, synthase sequences.
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<StructureSection load='1i18' size='340' side='right'caption='[[1i18]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1i18]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I18 FirstGlance]. <br>
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1I18 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with RBF as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I18 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RBF:RIBOFLAVIN'>RBF</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i18 OCA], [https://pdbe.org/1i18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i18 RCSB], [https://www.ebi.ac.uk/pdbsum/1i18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i18 ProSAT]</span></td></tr>
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The solution structure of the N-terminal domain of riboflavin synthase., Truffault V, Coles M, Diercks T, Abelmann K, Eberhardt S, Luttgen H, Bacher A, Kessler H, J Mol Biol. 2001 Jun 15;309(4):949-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11399071 11399071]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RISA_ECOLI RISA_ECOLI] Catalyzes the dismutation of two molecules of 6,7-dimethyl-8-ribityllumazine, resulting in the formation of riboflavin and 5-amino-6-(D-ribitylamino)uracil.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i1/1i18_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i18 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Riboflavin synthase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Abelmann K]]
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[[Category: Abelmann, K.]]
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[[Category: Bacher A]]
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[[Category: Bacher, A.]]
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[[Category: Coles M]]
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[[Category: Coles, M.]]
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[[Category: Diercks T]]
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[[Category: Diercks, T.]]
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[[Category: Eberhardt S]]
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[[Category: Eberhardt, S.]]
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[[Category: Kessler H]]
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[[Category: Kessler, H.]]
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[[Category: Luettgen H]]
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[[Category: Luettgen, H.]]
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[[Category: Truffault V]]
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[[Category: Truffault, V.]]
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[[Category: RBF]]
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[[Category: greek-key-barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 16:58:04 2007''
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Current revision

SOLUTION STRUCTURE OF THE N-TERMINAL DOMAIN OF RIBOFLAVIN SYNTHASE FROM E. COLI

PDB ID 1i18

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