1l5b

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{{Seed}}
 
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[[Image:1l5b.png|left|200px]]
 
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==DOMAIN-SWAPPED CYANOVIRIN-N DIMER==
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The line below this paragraph, containing "STRUCTURE_1l5b", creates the "Structure Box" on the page.
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<StructureSection load='1l5b' size='340' side='right'caption='[[1l5b]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1l5b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L5B FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
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{{STRUCTURE_1l5b| PDB=1l5b | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l5b OCA], [https://pdbe.org/1l5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l5b RCSB], [https://www.ebi.ac.uk/pdbsum/1l5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l5b ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CVN_NOSEL CVN_NOSEL] Mannose-binding lectin.<ref>PMID:9210678</ref> <ref>PMID:12678493</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l5/1l5b_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l5b ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the potent HIV-inactivating protein cyanovirin-N was previously found by NMR to be a monomer in solution and a domain-swapped dimer by X-ray crystallography. Here we demonstrate that, in solution, CV-N can exist both in monomeric and in domain-swapped dimeric form. The dimer is a metastable, kinetically trapped structure at neutral pH and room temperature. Based on orientational NMR constraints, we show that the domain-swapped solution dimer is similar to structures in two different crystal forms, exhibiting solely a small reorientation around the hinge region. Mutation of the single proline residue in the hinge to glycine significantly stabilizes the protein in both its monomeric and dimeric forms. By contrast, mutation of the neighboring serine to proline results in an exclusively dimeric protein, caused by a drastic destabilization of the monomer.
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===DOMAIN-SWAPPED CYANOVIRIN-N DIMER===
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The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures.,Barrientos LG, Louis JM, Botos I, Mori T, Han Z, O'Keefe BR, Boyd MR, Wlodawer A, Gronenborn AM Structure. 2002 May;10(5):673-86. PMID:12015150<ref>PMID:12015150</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12015150}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1l5b" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12015150 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12015150}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1L5B is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nostoc_ellipsosporum Nostoc ellipsosporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L5B OCA].
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==Reference==
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The domain-swapped dimer of cyanovirin-N is in a metastable folded state: reconciliation of X-ray and NMR structures., Barrientos LG, Louis JM, Botos I, Mori T, Han Z, O'Keefe BR, Boyd MR, Wlodawer A, Gronenborn AM, Structure. 2002 May;10(5):673-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12015150 12015150]
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[[Category: Nostoc ellipsosporum]]
[[Category: Nostoc ellipsosporum]]
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[[Category: Single protein]]
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[[Category: Barrientos LG]]
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[[Category: Barrientos, L G.]]
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[[Category: Botos I]]
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[[Category: Botos, I.]]
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[[Category: Boyd MR]]
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[[Category: Boyd, M R.]]
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[[Category: Gronenborn AM]]
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[[Category: Gronenborn, A M.]]
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[[Category: Han Z]]
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[[Category: Han, Z.]]
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[[Category: Louis JM]]
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[[Category: Keefe, B R.O.]]
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[[Category: Mori T]]
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[[Category: Louis, J M.]]
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[[Category: O'Keefe BR]]
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[[Category: Mori, T.]]
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[[Category: Wlodawer A]]
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[[Category: Wlodawer, A.]]
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[[Category: Cyanovirin-n]]
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[[Category: Domain-swapping]]
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[[Category: Gp120]]
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[[Category: Hiv-inactivating]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 11:44:07 2008''
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Current revision

DOMAIN-SWAPPED CYANOVIRIN-N DIMER

PDB ID 1l5b

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