1l9l

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{{Seed}}
 
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[[Image:1l9l.png|left|200px]]
 
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==GRANULYSIN FROM HUMAN CYTOLYTIC T LYMPHOCYTES==
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The line below this paragraph, containing "STRUCTURE_1l9l", creates the "Structure Box" on the page.
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<StructureSection load='1l9l' size='340' side='right'caption='[[1l9l]], [[Resolution|resolution]] 0.92&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1l9l]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1L9L FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 0.92&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EOH:ETHANOL'>EOH</scene>, <scene name='pdbligand=MPO:3[N-MORPHOLINO]PROPANE+SULFONIC+ACID'>MPO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1l9l| PDB=1l9l | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1l9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1l9l OCA], [https://pdbe.org/1l9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1l9l RCSB], [https://www.ebi.ac.uk/pdbsum/1l9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1l9l ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GNLY_HUMAN GNLY_HUMAN] Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l9/1l9l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1l9l ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Our crystal structure of granulysin suggests a mechanism for lysis of bacterial membranes by granulysin, a 74-residue basic protein from human cytolytic T lymphocyte and natural killer cells. We determined the initial crystal structure of selenomethionyl granulysin by MAD phasing at 2A resolution. We present the structure model refined using native diffraction data to 0.96A resolution. The five-helical bundle of granulysin resembles other "saposin folds" (such as NK-lysin). Positive charges distribute in a ring around the granulysin molecule, and one face has net positive charge. Sulfate ions bind near the segment of the molecule identified as most membrane-lytic and of highest hydrophobic moment. The ion locations may indicate granulysin's orientation of initial approach towards the membrane. The crystal packing reveals one way to pack a sheet of granulysin molecules at the cell surface for a concerted lysis effort. The energy of binding granulysin charges to the bacterial membrane could drive the subsequent lytic processes. The loosely packed core facilitates a hinge or scissors motion towards exposure of hydrophobic surface that we propose tunnels the granulysin into the fracturing target membrane.
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===GRANULYSIN FROM HUMAN CYTOLYTIC T LYMPHOCYTES===
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Granulysin crystal structure and a structure-derived lytic mechanism.,Anderson DH, Sawaya MR, Cascio D, Ernst W, Modlin R, Krensky A, Eisenberg D J Mol Biol. 2003 Jan 10;325(2):355-65. PMID:12488100<ref>PMID:12488100</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_12488100}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1l9l" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 12488100 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_12488100}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1L9L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1L9L OCA].
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==Reference==
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Granulysin crystal structure and a structure-derived lytic mechanism., Anderson DH, Sawaya MR, Cascio D, Ernst W, Modlin R, Krensky A, Eisenberg D, J Mol Biol. 2003 Jan 10;325(2):355-65. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12488100 12488100]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Anderson, D H.]]
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[[Category: Anderson DH]]
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[[Category: Cascio, D.]]
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[[Category: Cascio D]]
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[[Category: Eisenberg, D.]]
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[[Category: Eisenberg D]]
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[[Category: Ernst, W.]]
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[[Category: Ernst W]]
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[[Category: Krensky, A.]]
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[[Category: Krensky A]]
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[[Category: Modlin, R.]]
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[[Category: Modlin R]]
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[[Category: Sawaya, M R.]]
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[[Category: Sawaya MR]]
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[[Category: Granulysin]]
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[[Category: Membrane-lytic]]
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[[Category: Saposin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 12:01:58 2008''
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Current revision

GRANULYSIN FROM HUMAN CYTOLYTIC T LYMPHOCYTES

PDB ID 1l9l

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