1i4w

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(New page: 200px<br /><applet load="1i4w" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i4w, resolution 2.60&Aring;" /> '''THE CRYSTAL STRUCTUR...)
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[[Image:1i4w.gif|left|200px]]<br /><applet load="1i4w" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i4w, resolution 2.60&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION==
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Although it is commonly accepted that binding of mitochondrial, transcription factor sc-mtTFB to the mitochondrial RNA polymerase is, required for specific transcription initiation in Saccharomyces, cerevisiae, its precise role has remained undefined. In the present work, the crystal structure of sc-mtTFB has been determined to 2.6 A resolution., The protein consists of two domains, an N-terminal alpha/beta-domain and a, smaller domain made up of four alpha-helices. Contrary to previous, predictions, sc-mtTFB does not resemble Escherichia coli sigma-factors but, rather is structurally homologous to rRNA methyltransferase ErmC'. This, suggests that sc-mtTFB functions as an RNA-binding protein, an observation, standing in contradiction to the existing model, which proposed a direct, interaction of sc-mtTFB with the mitochondrial DNA promoter. Based on the, structure, we propose that the promoter specificity region is located on, the mitochondrial RNA polymerase and that binding of sc-mtTFB indirectly, mediates interaction of the core enzyme with the promoter site.
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<StructureSection load='1i4w' size='340' side='right'caption='[[1i4w]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1i4w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I4W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I4W FirstGlance]. <br>
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1I4W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with XE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I4W OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=XE:XENON'>XE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i4w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i4w OCA], [https://pdbe.org/1i4w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i4w RCSB], [https://www.ebi.ac.uk/pdbsum/1i4w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i4w ProSAT]</span></td></tr>
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Crystal structure of the transcription factor sc-mtTFB offers insights into mitochondrial transcription., Schubot FD, Chen CJ, Rose JP, Dailey TA, Dailey HA, Wang BC, Protein Sci. 2001 Oct;10(10):1980-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11567089 11567089]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MTF1_YEAST MTF1_YEAST] Mitochondrial transcription factor that confers selective promoter recognition on the core subunit of the yeast mitochondrial RNA polymerase. Interacts with DNA in a non-specific manner.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i4/1i4w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i4w ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Chen C-J]]
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[[Category: Chen, C.J.]]
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[[Category: Dailey HA]]
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[[Category: Dailey, H.A.]]
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[[Category: Dailey TA]]
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[[Category: Dailey, T.A.]]
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[[Category: Rose JP]]
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[[Category: Rose, J.P.]]
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[[Category: Schubot FD]]
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[[Category: Schubot, F.D.]]
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[[Category: Wang B-C]]
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[[Category: Wang, B.C.]]
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[[Category: XE]]
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[[Category: mitochondrial transcription factor]]
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[[Category: transcription initiation]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:03:11 2007''
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Current revision

THE CRYSTAL STRUCTURE OF THE TRANSCRIPTION FACTOR SC-MTTFB OFFERS INTRIGUING INSIGHTS INTO MITOCHONDRIAL TRANSCRIPTION

PDB ID 1i4w

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