1i5c

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(New page: 200px<br /><applet load="1i5c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i5c, resolution 1.90&Aring;" /> '''STRUCTURE OF CHEA DO...)
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[[Image:1i5c.gif|left|200px]]<br /><applet load="1i5c" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i5c, resolution 1.90&Aring;" />
 
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'''STRUCTURE OF CHEA DOMAIN P4 IN COMPLEX WITH ADP'''<br />
 
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==Overview==
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==STRUCTURE OF CHEA DOMAIN P4 IN COMPLEX WITH ADP==
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To probe the structural basis for protein histidine kinase (PHK) catalytic, activity and the prospects for PHK-specific inhibitor design, we report, the crystal structures for the nucleotide binding domain of Thermotoga, maritima CheA with ADP and three ATP analogs (ADPNP, ADPCP and TNP-ATP), bound with either Mg(2+) or Mn(2+). The conformation of ADPNP bound to, CheA and related ATPases differs from that reported in the ADPNP complex, of PHK EnvZ. Interactions of the active site with the nucleotide, gamma-phosphate and its associated Mg(2+) ion are linked to conformational, changes in an ATP-lid that could mediate recognition of the substrate, domain. The inhibitor TNP-ATP binds CheA with its phosphates in a, nonproductive conformation and its adenine and trinitrophenyl groups in, two adjacent binding pockets. The trinitrophenyl interaction may be, exploited for designing CheA-targeted drugs that would not interfere with, host ATPases.
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<StructureSection load='1i5c' size='340' side='right'caption='[[1i5c]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1i5c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I5C FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i5c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5c OCA], [https://pdbe.org/1i5c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i5c RCSB], [https://www.ebi.ac.uk/pdbsum/1i5c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i5c ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHEA_THEMA CHEA_THEMA] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. CheA is autophosphorylated; it can transfer its phosphate group to either CheB or CheY (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/1i5c_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i5c ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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To probe the structural basis for protein histidine kinase (PHK) catalytic activity and the prospects for PHK-specific inhibitor design, we report the crystal structures for the nucleotide binding domain of Thermotoga maritima CheA with ADP and three ATP analogs (ADPNP, ADPCP and TNP-ATP) bound with either Mg(2+) or Mn(2+). The conformation of ADPNP bound to CheA and related ATPases differs from that reported in the ADPNP complex of PHK EnvZ. Interactions of the active site with the nucleotide gamma-phosphate and its associated Mg(2+) ion are linked to conformational changes in an ATP-lid that could mediate recognition of the substrate domain. The inhibitor TNP-ATP binds CheA with its phosphates in a nonproductive conformation and its adenine and trinitrophenyl groups in two adjacent binding pockets. The trinitrophenyl interaction may be exploited for designing CheA-targeted drugs that would not interfere with host ATPases.
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==About this Structure==
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Nucleotide binding by the histidine kinase CheA.,Bilwes AM, Quezada CM, Croal LR, Crane BR, Simon MI Nat Struct Biol. 2001 Apr;8(4):353-60. PMID:11276258<ref>PMID:11276258</ref>
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1I5C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I5C OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Nucleotide binding by the histidine kinase CheA., Bilwes AM, Quezada CM, Croal LR, Crane BR, Simon MI, Nat Struct Biol. 2001 Apr;8(4):353-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11276258 11276258]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1i5c" style="background-color:#fffaf0;"></div>
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[[Category: Thermotoga maritima]]
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[[Category: Bilwes, A.M.]]
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[[Category: Crane, B.R.]]
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[[Category: Croal, L.R.]]
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[[Category: Quezada, C.M.]]
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[[Category: Simon, M.I.]]
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[[Category: ADP]]
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[[Category: beta-alpha sandwich]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:04:05 2007''
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==See Also==
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*[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermotoga maritima]]
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[[Category: Bilwes AM]]
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[[Category: Crane BR]]
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[[Category: Croal LR]]
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[[Category: Quezada CM]]
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[[Category: Simon MI]]

Current revision

STRUCTURE OF CHEA DOMAIN P4 IN COMPLEX WITH ADP

PDB ID 1i5c

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