1i5l

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(New page: 200px<br /><applet load="1i5l" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i5l, resolution 2.75&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1i5l.gif|left|200px]]<br /><applet load="1i5l" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i5l, resolution 2.75&Aring;" />
 
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'''CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH SHORT POLY-U RNA'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH SHORT POLY-U RNA==
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Eukaryotic Sm and Sm-like proteins associate with RNA to form the core, domain of ribonucleoprotein particles involved in pre-mRNA splicing and, other processes. Recently, putative Sm proteins of unknown function have, been identified in ARCHAEA: We show by immunoprecipitation experiments, that the two Sm proteins present in Archaeoglobus fulgidus (AF-Sm1 and, AF-Sm2) associate with RNase P RNA in vivo, suggesting a role in tRNA, processing. The AF-Sm1 protein also interacts specifically with, oligouridylate in vitro. We have solved the crystal structures of this, protein and a complex with RNA. AF-Sm1 forms a seven-membered ring, with, the RNA interacting inside the central cavity on one face of the, doughnut-shaped complex. The bases are bound via stacking and specific, hydrogen bonding contacts in pockets lined by residues highly conserved in, archaeal and eukaryotic Sm proteins, while the phosphates remain solvent, accessible. A comparison with the structures of human Sm protein dimers, reveals closely related monomer folds and intersubunit contacts, indicating that the architecture of the Sm core domain and RNA binding, have been conserved during evolution.
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<StructureSection load='1i5l' size='340' side='right'caption='[[1i5l]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1i5l]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I5L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I5L FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=URI:URIDINE'>URI</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i5l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i5l OCA], [https://pdbe.org/1i5l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i5l RCSB], [https://www.ebi.ac.uk/pdbsum/1i5l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i5l ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RUXX_ARCFU RUXX_ARCFU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i5/1i5l_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i5l ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1I5L is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus] with URI as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I5L OCA].
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*[[Sm-like protein 3D structures|Sm-like protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex., Toro I, Thore S, Mayer C, Basquin J, Seraphin B, Suck D, EMBO J. 2001 May 1;20(9):2293-303. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11331594 11331594]
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[[Category: Archaeoglobus fulgidus]]
[[Category: Archaeoglobus fulgidus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Basquin, J.]]
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[[Category: Basquin J]]
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[[Category: Mayer, C.]]
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[[Category: Mayer C]]
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[[Category: Seraphin, B.]]
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[[Category: Seraphin B]]
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[[Category: Suck, D.]]
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[[Category: Suck D]]
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[[Category: Thore, S.]]
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[[Category: Thore S]]
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[[Category: Toro, I.]]
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[[Category: Toro I]]
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[[Category: URI]]
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[[Category: core snrnp domain]]
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[[Category: rna binding protein]]
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[[Category: single-stranded rna binding protein]]
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[[Category: sm]]
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[[Category: snrnp]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:04:28 2007''
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Current revision

CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH SHORT POLY-U RNA

PDB ID 1i5l

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