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1i6f

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(New page: 200px<br /><applet load="1i6f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i6f" /> '''NMR SOLUTION STRUCTURE OF THE INSECT-SPECIFI...)
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[[Image:1i6f.jpg|left|200px]]<br /><applet load="1i6f" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1i6f" />
 
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'''NMR SOLUTION STRUCTURE OF THE INSECT-SPECIFIC NEUROTOXIN VARIANT 5 (CSE-V5) FROM THE SCORPION CENTRUROIDES SCULPTURATUS EWING'''<br />
 
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==Overview==
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==NMR SOLUTION STRUCTURE OF THE INSECT-SPECIFIC NEUROTOXIN VARIANT 5 (CSE-V5) FROM THE SCORPION CENTRUROIDES SCULPTURATUS EWING==
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We report the high-resolution solution structure of the 6.3 kDa neurotoxic, protein CsE-v5 from the scorpion Centruroides sculpturatus Ewing (CsE, range southwestern U.S.). This protein is the second example of an Old, World-like neurotoxin isolated from the venom of this New World scorpion., However, unlike CsE-V, which is the first Old World-like toxin isolated, and shows both anti-insect and anti-mammal activity, CsE-v5 shows high, specificity for insect sodium channels. Sequence-specific proton NMR, assignments and distance and angle constraints were obtained from 600 MHz, 2D-NMR data. Distance geometry and dynamical simulated annealing, refinements were performed to produce a final family of 20 structures, without constraint violations, along with an energy-minimized average, structure. The protein structure is well-defined (0.66 and 0.97 D rmsd for, backbone and all heavy atoms, respectively) with a compact hydrophobic, core and several extending loops. A large hydrophobic patch, containing, four aromatic rings and other aliphatic residues, makes up a large area of, one side of the protein. CsE-v5 shows secondary structural features, characteristic of long-chain scorpion toxins: a two and a half-turn, alpha-helix, a three-strand antiparallel beta-sheet, and four beta-turns., Among the proteins studied to date from the CsE venom, CsE-v5 is the most, compact protein with nearly 50% of the amide protons having long exchange, lifetimes, but CsE-v5 is unusual in that it has loop structures similar to, both Old and New World toxins. Further, it also lacks prolines in its, C-terminal 14 residues. It shows some important differences with respect, to CsE-V not only in its primary sequence, but also in its electrostatic, potential surface, especially around areas in register with residues 8, 9, 17, 18, 32, 43, and 57. The loss of anti-mammal activity in CsE-v5 and the, differences in its anti-insect activity compared to that of other proteins, such as CsE-V, v1, and v3 from this New World scorpion may be related to, residue variations at these locations.
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<StructureSection load='1i6f' size='340' side='right'caption='[[1i6f]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1i6f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Centruroides_sculpturatus Centruroides sculpturatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I6F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I6F FirstGlance]. <br>
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1I6F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_sculpturatus Centruroides sculpturatus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I6F OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i6f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i6f OCA], [https://pdbe.org/1i6f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i6f RCSB], [https://www.ebi.ac.uk/pdbsum/1i6f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i6f ProSAT]</span></td></tr>
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==Reference==
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</table>
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Solution structure of an insect-specific neurotoxin from the New World scorpion Centruroides sculpturatus Ewing., Jablonsky MJ, Jackson PL, Krishna NR, Biochemistry. 2001 Jul 27;40(28):8273-82. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11444973 11444973]
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== Function ==
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[https://www.uniprot.org/uniprot/SCX5_CENSC SCX5_CENSC]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i6/1i6f_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i6f ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Centruroides sculpturatus]]
[[Category: Centruroides sculpturatus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Jablonsky, M.J.]]
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[[Category: Jablonsky MJ]]
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[[Category: Jackson, P.L.]]
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[[Category: Jackson PL]]
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[[Category: Krishna, N.R.]]
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[[Category: Krishna NR]]
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[[Category: alpha helix]]
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[[Category: beta sheet]]
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[[Category: disulfide linkages]]
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[[Category: neurotoxin]]
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[[Category: scorpion]]
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[[Category: sodium channel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:05:29 2007''
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Current revision

NMR SOLUTION STRUCTURE OF THE INSECT-SPECIFIC NEUROTOXIN VARIANT 5 (CSE-V5) FROM THE SCORPION CENTRUROIDES SCULPTURATUS EWING

PDB ID 1i6f

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