1lmn

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{{Seed}}
 
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[[Image:1lmn.png|left|200px]]
 
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==THE REFINED CRYSTAL STRUCTURE OF LYSOZYME FROM THE RAINBOW TROUT (ONCORHYNCHUS MYKISS)==
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The line below this paragraph, containing "STRUCTURE_1lmn", creates the "Structure Box" on the page.
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<StructureSection load='1lmn' size='340' side='right'caption='[[1lmn]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1lmn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1LMN FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1lmn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1lmn OCA], [https://pdbe.org/1lmn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1lmn RCSB], [https://www.ebi.ac.uk/pdbsum/1lmn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1lmn ProSAT]</span></td></tr>
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{{STRUCTURE_1lmn| PDB=1lmn | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LYSC2_ONCMY LYSC2_ONCMY] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has antibacterial activity against the Gram positive bacterium P.citreus. Has no antibacterial activity against the Gram negative bacteria E.coli and Y.ruckeri. Does not have hemolytic activity against trout erythrocytes.<ref>PMID:15142536</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/lm/1lmn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1lmn ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Lysozymes (E.C. 3.2.1.17) are well characterized ubiquitous enzymes that have an antibacterial effect. The lysozymes from rainbow trout (RBTL) (Oncorhynchus mykiss) could be particularly interesting in aquaculture since they show higher activity than egg-white lysozyme and lysozymes from other fish species against a variety of pathogenic bacteria. Two lysozymes, I and II, differing only in a single amino acid, were purified from the kidney of rainbow trout and shown to belong to the c-type class of lysozymes. The type II form was shown to be much more potent against a variety of bacteria than the type I enzyme. We have grown crystals from a mixture containing about 80% type I and 20% type II lysozyme from rainbow trout, and solved the X-ray crystal structure. The crystals are trigonal with a = 76.68, c = 54.46 A and space group P3(1)21. The phase problem was solved by the molecular-replacement method, and the structure was refined to an R-factor of 17.4% using data to 1.8 A resolution. The crystal structure shows that the three-dimensional structure of rainbow trout lysozyme is very similar to the previously solved structures of other c-type lysozymes. The single polypeptide of 129 amino acids is folded into two domains separated by a deep cleft which contains the active site. Secondary-structure elements, four alpha-helices and a three-stranded beta-sheet, are located in the same sequential positions as in the hen, turkey and human enzymes. The beta-sheet is found to be common for structures of both c- and g-type lysozymes. We suggest that differences in antibiotic activity of the two forms of RBTL are probably due to small differences in the hydophobicity of a small surface region.
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===THE REFINED CRYSTAL STRUCTURE OF LYSOZYME FROM THE RAINBOW TROUT (ONCORHYNCHUS MYKISS)===
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Refined crystal structure of lysozyme from the rainbow trout (Oncorhynchus mykiss).,Karlsen S, Eliassen BE, Hansen LK, Larsen RL, Riise BW, Smalas AO, Hough E, Grinde B Acta Crystallogr D Biol Crystallogr. 1995 May 1;51(Pt 3):354-67. PMID:15299303<ref>PMID:15299303</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 1lmn" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_15299303}}, adds the Publication Abstract to the page
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 15299303 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15299303}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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1LMN is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Oncorhynchus_mykiss Oncorhynchus mykiss]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LMN OCA].
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==Reference==
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Refined crystal structure of lysozyme from the rainbow trout (Oncorhynchus mykiss)., Karlsen S, Eliassen BE, Hansen LK, Larsen RL, Riise BW, Smalas AO, Hough E, Grinde B, Acta Crystallogr D Biol Crystallogr. 1995 May 1;51(Pt 3):354-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299303 15299303]
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[[Category: Lysozyme]]
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[[Category: Oncorhynchus mykiss]]
[[Category: Oncorhynchus mykiss]]
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[[Category: Single protein]]
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[[Category: Hough E]]
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[[Category: Hough, E.]]
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[[Category: Karlsen S]]
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[[Category: Karlsen, S.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:26:12 2008''
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Current revision

THE REFINED CRYSTAL STRUCTURE OF LYSOZYME FROM THE RAINBOW TROUT (ONCORHYNCHUS MYKISS)

PDB ID 1lmn

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