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- | {{Seed}} | |
- | [[Image:2r19.png|left|200px]] | |
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- | <!--
| + | ==Crystal structure of the periplasmic lipopolysaccharide transport protein LptA (YhbN), orthorhombic form== |
- | The line below this paragraph, containing "STRUCTURE_2r19", creates the "Structure Box" on the page.
| + | <StructureSection load='2r19' size='340' side='right'caption='[[2r19]], [[Resolution|resolution]] 2.16Å' scene=''> |
- | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
- | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[2r19]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R19 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R19 FirstGlance]. <br> |
- | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.16Å</td></tr> |
- | -->
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r19 OCA], [https://pdbe.org/2r19 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r19 RCSB], [https://www.ebi.ac.uk/pdbsum/2r19 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r19 ProSAT]</span></td></tr> |
- | {{STRUCTURE_2r19| PDB=2r19 | SCENE= }}
| + | </table> |
- | | + | == Function == |
- | ===Crystal structure of the periplasmic lipopolysaccharide transport protein LptA (YhbN), orthorhombic form===
| + | [https://www.uniprot.org/uniprot/LPTA_ECOLI LPTA_ECOLI] Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. May form a bridge between the inner membrane and the outer membrane, via interactions with LptC and LptD, thereby facilitating LPS transfer across the periplasm.[HAMAP-Rule:MF_01914]<ref>PMID:16765569</ref> <ref>PMID:17056748</ref> <ref>PMID:18424520</ref> <ref>PMID:18480051</ref> <ref>PMID:21169485</ref> |
- | | + | == Evolutionary Conservation == |
- | | + | [[Image:Consurf_key_small.gif|200px|right]] |
- | <!-- | + | Check<jmol> |
- | The line below this paragraph, {{ABSTRACT_PUBMED_18534617}}, adds the Publication Abstract to the page
| + | <jmolCheckbox> |
- | (as it appears on PubMed at http://www.pubmed.gov), where 18534617 is the PubMed ID number.
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/2r19_consurf.spt"</scriptWhenChecked> |
- | -->
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
- | {{ABSTRACT_PUBMED_18534617}}
| + | <text>to colour the structure by Evolutionary Conservation</text> |
- | | + | </jmolCheckbox> |
- | ==About this Structure== | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r19 ConSurf]. |
- | 2R19 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R19 OCA].
| + | <div style="clear:both"></div> |
- | | + | == References == |
- | ==Reference== | + | <references/> |
- | Novel structure of the conserved gram-negative lipopolysaccharide transport protein a and mutagenesis analysis., Suits MD, Sperandeo P, Deho G, Polissi A, Jia Z, J Mol Biol. 2008 Jul 11;380(3):476-88. Epub 2008 Apr 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18534617 18534617]
| + | __TOC__ |
- | [[Category: Escherichia coli k12]] | + | </StructureSection> |
- | [[Category: Single protein]] | + | [[Category: Escherichia coli K-12]] |
- | [[Category: BSGI, Montreal-Kingston Bacterial Structural Genomics Initiative.]] | + | [[Category: Large Structures]] |
- | [[Category: Jia, Z.]] | + | [[Category: Jia Z]] |
- | [[Category: Polissi, A.]] | + | [[Category: Polissi A]] |
- | [[Category: Suits, M D.L.]] | + | [[Category: Suits MDL]] |
- | [[Category: Beta-jellyroll]] | + | |
- | [[Category: Beta-taco]]
| + | |
- | [[Category: Bsgi]]
| + | |
- | [[Category: Mainly beta]] | + | |
- | [[Category: Montreal-kingston bacterial structural genomics initiative]] | + | |
- | [[Category: Periplasm]] | + | |
- | [[Category: Structural genomic]] | + | |
- | [[Category: Transport protein]] | + | |
- | | + | |
- | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 21:36:03 2008''
| + | |
| Structural highlights
Function
LPTA_ECOLI Involved in the assembly of lipopolysaccharide (LPS). Required for the translocation of LPS from the inner membrane to the outer membrane. May form a bridge between the inner membrane and the outer membrane, via interactions with LptC and LptD, thereby facilitating LPS transfer across the periplasm.[HAMAP-Rule:MF_01914][1] [2] [3] [4] [5]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Sperandeo P, Pozzi C, Deho G, Polissi A. Non-essential KDO biosynthesis and new essential cell envelope biogenesis genes in the Escherichia coli yrbG-yhbG locus. Res Microbiol. 2006 Jul-Aug;157(6):547-58. Epub 2006 Feb 9. PMID:16765569 doi:10.1016/j.resmic.2005.11.014
- ↑ Sperandeo P, Cescutti R, Villa R, Di Benedetto C, Candia D, Deho G, Polissi A. Characterization of lptA and lptB, two essential genes implicated in lipopolysaccharide transport to the outer membrane of Escherichia coli. J Bacteriol. 2007 Jan;189(1):244-53. Epub 2006 Oct 20. PMID:17056748 doi:http://dx.doi.org/10.1128/JB.01126-06
- ↑ Sperandeo P, Lau FK, Carpentieri A, De Castro C, Molinaro A, Deho G, Silhavy TJ, Polissi A. Functional analysis of the protein machinery required for transport of lipopolysaccharide to the outer membrane of Escherichia coli. J Bacteriol. 2008 Jul;190(13):4460-9. Epub 2008 Apr 18. PMID:18424520 doi:JB.00270-08
- ↑ Tran AX, Trent MS, Whitfield C. The LptA protein of Escherichia coli is a periplasmic lipid A-binding protein involved in the lipopolysaccharide export pathway. J Biol Chem. 2008 Jul 18;283(29):20342-9. doi: 10.1074/jbc.M802503200. Epub 2008 , May 14. PMID:18480051 doi:http://dx.doi.org/10.1074/jbc.M802503200
- ↑ Sperandeo P, Villa R, Martorana AM, Samalikova M, Grandori R, Deho G, Polissi A. New insights into the Lpt machinery for lipopolysaccharide transport to the cell surface: LptA-LptC interaction and LptA stability as sensors of a properly assembled transenvelope complex. J Bacteriol. 2011 Mar;193(5):1042-53. doi: 10.1128/JB.01037-10. Epub 2010 Dec 17. PMID:21169485 doi:http://dx.doi.org/10.1128/JB.01037-10
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