1ibs

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(New page: 200px<br /><applet load="1ibs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ibs, resolution 2.8&Aring;" /> '''PHOSPHORIBOSYLDIPHOSP...)
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[[Image:1ibs.jpg|left|200px]]<br /><applet load="1ibs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ibs, resolution 2.8&Aring;" />
 
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'''PHOSPHORIBOSYLDIPHOSPHATE SYNTHETASE IN COMPLEX WITH CADMIUM IONS'''<br />
 
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==Overview==
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==PHOSPHORIBOSYLDIPHOSPHATE SYNTHETASE IN COMPLEX WITH CADMIUM IONS==
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The binding sites for the two cations essential for the catalytic function, of 5-phospho-D-ribosyl alpha-1-diphosphate (PRPP) synthases have been, identified from the structure of the Bacillus subtilis, phosphoribosyldiphosphate synthetase (PRPPsase) with bound Cd(2+). The, structure determined from X-ray diffraction data to 2.8-A resolution, reveals the same hexameric arrangement of the subunits that was observed, in the complexes of the enzyme with the activator sulfate and the, allosteric inhibitor ADP. Two cation binding sites were localized in each, of the two domains of the subunits that compose the hexamer; each domain, of the subunit has an associated cation. In addition to the bound Cd(2+), the Cd(2+)-PRPPsase structure contains a sulfate ion in the regulatory, site, a sulfate ion at the ribose-5-phosphate binding site, and an AMP, moiety at the ATP binding site. Comparison of the Cd(2+)-PRPPsase to the, structures of the PRPPsase complexed with sulfate and mADP reveals the, structural rearrangement induced by the binding of the free cation, which, is essential for the initiation of the reaction. The comparison to the, cPRPP complex of glutamine phosphoribosylpyrophosphate amidotransferase, from Escherichia coli, a type I phosphoribosyltransferase, provided, information about the binding of PRPP. This strongly indicates that the, binding of both substrates must lead to a stabilized conformation of the, loop region, which remains unresolved in the known PRPPsase complex, structures.
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<StructureSection load='1ibs' size='340' side='right'caption='[[1ibs]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ibs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IBS FirstGlance]. <br>
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1IBS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with SO4, CD and ABM as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribose-phosphate_diphosphokinase Ribose-phosphate diphosphokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.6.1 2.7.6.1] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IBS OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ABM:METHYL+PHOSPHONIC+ACID+ADENOSINE+ESTER'>ABM</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ibs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ibs OCA], [https://pdbe.org/1ibs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ibs RCSB], [https://www.ebi.ac.uk/pdbsum/1ibs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ibs ProSAT]</span></td></tr>
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Binding of cations in Bacillus subtilis phosphoribosyldiphosphate synthetase and their role in catalysis., Eriksen TA, Kadziola A, Larsen S, Protein Sci. 2002 Feb;11(2):271-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11790837 11790837]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KPRS_BACSU KPRS_BACSU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ib/1ibs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ibs ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Ribose-phosphate diphosphokinase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Eriksen TA]]
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[[Category: Eriksen, T.A.]]
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[[Category: Kadziola A]]
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[[Category: Kadziola, A.]]
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[[Category: Larsen S]]
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[[Category: Larsen, S.]]
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[[Category: ABM]]
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[[Category: CD]]
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[[Category: SO4]]
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[[Category: domain duplication]]
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[[Category: open alpha beta structure]]
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[[Category: phosphoribosyltransferase type i fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:16:04 2007''
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Current revision

PHOSPHORIBOSYLDIPHOSPHATE SYNTHETASE IN COMPLEX WITH CADMIUM IONS

PDB ID 1ibs

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