1igq

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(New page: 200px<br /><applet load="1igq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1igq, resolution 1.7&Aring;" /> '''C-terminal Domain of ...)
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[[Image:1igq.gif|left|200px]]<br /><applet load="1igq" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1igq, resolution 1.7&Aring;" />
 
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'''C-terminal Domain of Transcriptional Repressor Protein KorB'''<br />
 
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==Overview==
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==C-terminal Domain of Transcriptional Repressor Protein KorB==
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KorB is a regulatory protein encoded by the conjugative plasmid RP4 and a, member of the ParB family of bacterial partitioning proteins. The protein, regulates the expression of plasmid genes whose products are involved in, replication, transfer, and stable inheritance of RP4 by binding to, palindromic 13-bp DNA sequences (5'-TTTAGC(G/C)GCTAAA-3') present 12 times, in the 60-kb plasmid. Here we report the crystal structure of KorB-C, the, C-terminal domain of KorB comprising residues 297-358. The structure of, KorB-C was solved in two crystal forms. Quite unexpectedly, we find that, KorB-C shows a fold closely resembling the Src homology 3 (SH3) domain, a, fold well known from proteins involved in eukaryotic signal transduction., From the arrangement of molecules in the asymmetric unit, it is concluded, that two molecules form a functionally relevant dimer. The detailed, analysis of the dimer interface and a chemical cross-linking study suggest, that the C-terminal domain is responsible for stabilizing the dimeric form, of KorB in solution to facilitate binding to the palindromic operator, sequence. The KorB-C crystal structure extends the range of, protein-protein interactions known to be promoted by SH3 and SH3-like, domains.
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<StructureSection load='1igq' size='340' side='right'caption='[[1igq]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1igq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IGQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IGQ FirstGlance]. <br>
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1IGQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IGQ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1igq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1igq OCA], [https://pdbe.org/1igq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1igq RCSB], [https://www.ebi.ac.uk/pdbsum/1igq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1igq ProSAT]</span></td></tr>
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==Reference==
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</table>
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An Src homology 3-like domain is responsible for dimerization of the repressor protein KorB encoded by the promiscuous IncP plasmid RP4., Delbruck H, Ziegelin G, Lanka E, Heinemann U, J Biol Chem. 2002 Feb 8;277(6):4191-8. Epub 2001 Nov 15. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11711548 11711548]
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== Function ==
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[https://www.uniprot.org/uniprot/KORB2_ECOLX KORB2_ECOLX] In conjunction with KorA, inhibits the transcription of the kilA, trfA and korAB operons. Is also involved in the negative control of the kilB operon.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ig/1igq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1igq ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Delbruck, H.]]
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[[Category: Delbruck H]]
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[[Category: Heinemann, U.]]
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[[Category: Heinemann U]]
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[[Category: dimerization domain]]
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[[Category: sh3 domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:23:14 2007''
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Current revision

C-terminal Domain of Transcriptional Repressor Protein KorB

PDB ID 1igq

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