1ihj

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(New page: 200px<br /><applet load="1ihj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ihj, resolution 1.8&Aring;" /> '''Crystal Structure of ...)
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[[Image:1ihj.jpg|left|200px]]<br /><applet load="1ihj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ihj, resolution 1.8&Aring;" />
 
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'''Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide'''<br />
 
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==Overview==
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==Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide==
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In Drosophila, phototransduction is mediated by G(q)-activation of, phospholipase C and is a well studied model system for understanding the, kinetics of signal initiation, propagation and termination controlled by G, proteins. The proper intracellular targeting and spatial arrangement of, most proteins involved in fly phototransduction require the multi-domain, scaffolding protein InaD, composed almost entirely of five PDZ domains, which independently bind various proteins including NorpA, the relevant, phospho lipase C-beta isozyme. We have determined the crystal structure of, the N-terminal PDZ domain of InaD bound to a peptide corresponding to the, C-terminus of NorpA to 1.8 A resolution. The structure highlights an, intermolecular disulfide bond necessary for high affinity interaction as, determined by both in vitro and in vivo studies. Since other proteins also, possess similar, cysteine-containing consensus sequences for binding PDZ, domains, this disulfide-mediated 'dock-and-lock' interaction of PDZ, domains with their ligands may be a relatively ubiquitous mode of, coordinating signaling pathways.
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<StructureSection load='1ihj' size='340' side='right'caption='[[1ihj]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ihj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IHJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ihj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ihj OCA], [https://pdbe.org/1ihj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ihj RCSB], [https://www.ebi.ac.uk/pdbsum/1ihj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ihj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/INAD_DROME INAD_DROME] Involved in the negative feedback regulation of the light-activated signaling cascade in photoreceptors through a calcium-mediated process. Interacts with tetrapeptide ligand located in C-terminal sequence of 3 key components of the visual cascade, tethering them and forming a macromolecular signaling phototransduction complex.<ref>PMID:7826638</ref> <ref>PMID:11342563</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ih/1ihj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ihj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In Drosophila, phototransduction is mediated by G(q)-activation of phospholipase C and is a well studied model system for understanding the kinetics of signal initiation, propagation and termination controlled by G proteins. The proper intracellular targeting and spatial arrangement of most proteins involved in fly phototransduction require the multi-domain scaffolding protein InaD, composed almost entirely of five PDZ domains, which independently bind various proteins including NorpA, the relevant phospho lipase C-beta isozyme. We have determined the crystal structure of the N-terminal PDZ domain of InaD bound to a peptide corresponding to the C-terminus of NorpA to 1.8 A resolution. The structure highlights an intermolecular disulfide bond necessary for high affinity interaction as determined by both in vitro and in vivo studies. Since other proteins also possess similar, cysteine-containing consensus sequences for binding PDZ domains, this disulfide-mediated 'dock-and-lock' interaction of PDZ domains with their ligands may be a relatively ubiquitous mode of coordinating signaling pathways.
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==About this Structure==
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Functional relevance of the disulfide-linked complex of the N-terminal PDZ domain of InaD with NorpA.,Kimple ME, Siderovski DP, Sondek J EMBO J. 2001 Aug 15;20(16):4414-22. PMID:11500369<ref>PMID:11500369</ref>
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1IHJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Active as [http://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IHJ OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Functional relevance of the disulfide-linked complex of the N-terminal PDZ domain of InaD with NorpA., Kimple ME, Siderovski DP, Sondek J, EMBO J. 2001 Aug 15;20(16):4414-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11500369 11500369]
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</div>
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<div class="pdbe-citations 1ihj" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
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[[Category: Phospholipase C]]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Kimple ME]]
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[[Category: Kimple, M.E.]]
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[[Category: Siderovski DP]]
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[[Category: Siderovski, D.P.]]
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[[Category: Sondek J]]
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[[Category: Sondek, J.]]
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[[Category: intermolecular disulfide bond]]
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[[Category: pdz domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:24:05 2007''
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Current revision

Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide

PDB ID 1ihj

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