1ije

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(New page: 200px<br /><applet load="1ije" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ije, resolution 2.40&Aring;" /> '''Nucleotide Exchange ...)
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[[Image:1ije.jpg|left|200px]]<br /><applet load="1ije" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ije, resolution 2.40&Aring;" />
 
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'''Nucleotide Exchange Intermediates in the eEF1A-eEF1Ba Complex'''<br />
 
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==Overview==
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==Nucleotide Exchange Intermediates in the eEF1A-eEF1Ba Complex==
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In the elongation cycle of protein biosynthesis, the nucleotide exchange, factor eEF1Balpha catalyzes the exchange of GDP bound to the G-protein, eEF1A, for GTP. To obtain more information about the recently solved, eEF1A-eEF1Balpha structure, we determined the structures of the, eEF1A-eEF1Balpha-GDP-Mg2+, eEF1A-eEF1Balpha-GDP and eEF1A-eEF1Balpha-GDPNP, complexes at 3.0, 2.4 and 2.05 A resolution, respectively. Minor changes, specifically around the nucleotide binding site, in eEF1A and eEF1Balpha, are consistent with in vivo data. The base, sugar and alpha-phosphate bind, as in other known nucleotide G-protein complexes, whereas the beta- and, gamma-phosphates are disordered. A mutation of Lys 205 in eEF1Balpha that, inserts into the Mg2+ binding site of eEF1A is lethal. This together with, the structures emphasizes the essential role of Mg2+ in nucleotide, exchange in the eEF1A-eEF1Balpha complex.
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<StructureSection load='1ije' size='340' side='right'caption='[[1ije]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ije]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IJE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ije FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ije OCA], [https://pdbe.org/1ije PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ije RCSB], [https://www.ebi.ac.uk/pdbsum/1ije PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ije ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/EF1A_YEAST EF1A_YEAST]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ij/1ije_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ije ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In the elongation cycle of protein biosynthesis, the nucleotide exchange factor eEF1Balpha catalyzes the exchange of GDP bound to the G-protein, eEF1A, for GTP. To obtain more information about the recently solved eEF1A-eEF1Balpha structure, we determined the structures of the eEF1A-eEF1Balpha-GDP-Mg2+, eEF1A-eEF1Balpha-GDP and eEF1A-eEF1Balpha-GDPNP complexes at 3.0, 2.4 and 2.05 A resolution, respectively. Minor changes, specifically around the nucleotide binding site, in eEF1A and eEF1Balpha are consistent with in vivo data. The base, sugar and alpha-phosphate bind as in other known nucleotide G-protein complexes, whereas the beta- and gamma-phosphates are disordered. A mutation of Lys 205 in eEF1Balpha that inserts into the Mg2+ binding site of eEF1A is lethal. This together with the structures emphasizes the essential role of Mg2+ in nucleotide exchange in the eEF1A-eEF1Balpha complex.
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==About this Structure==
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Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex.,Andersen GR, Valente L, Pedersen L, Kinzy TG, Nyborg J Nat Struct Biol. 2001 Jun;8(6):531-4. PMID:11373622<ref>PMID:11373622</ref>
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1IJE is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with G25 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IJE OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structures of nucleotide exchange intermediates in the eEF1A-eEF1Balpha complex., Andersen GR, Valente L, Pedersen L, Kinzy TG, Nyborg J, Nat Struct Biol. 2001 Jun;8(6):531-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11373622 11373622]
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</div>
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[[Category: Protein complex]]
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<div class="pdbe-citations 1ije" style="background-color:#fffaf0;"></div>
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Andersen, G.R.]]
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[[Category: Kinzy, T.G.]]
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[[Category: Nyborg, J.]]
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[[Category: Pedersen, L.]]
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[[Category: Valente, L.]]
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[[Category: G25]]
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[[Category: protein complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:26:50 2007''
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==See Also==
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*[[Elongation factor 3D structures|Elongation factor 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Andersen GR]]
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[[Category: Kinzy TG]]
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[[Category: Nyborg J]]
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[[Category: Pedersen L]]
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[[Category: Valente L]]

Current revision

Nucleotide Exchange Intermediates in the eEF1A-eEF1Ba Complex

PDB ID 1ije

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