1in8

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(New page: 200px<br /><applet load="1in8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1in8, resolution 1.90&Aring;" /> '''THERMOTOGA MARITIMA ...)
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[[Image:1in8.jpg|left|200px]]<br /><applet load="1in8" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1in8, resolution 1.90&Aring;" />
 
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'''THERMOTOGA MARITIMA RUVB T158V'''<br />
 
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==Overview==
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==THERMOTOGA MARITIMA RUVB T158V==
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The RuvB hexamer is the chemomechanical motor of the RuvAB complex that, migrates Holliday junction branch-points in DNA recombination and the, rescue of stalled DNA replication forks. The 1.6 A crystal structure of, Thermotoga maritima RuvB together with five mutant structures reveal that, RuvB is an ATPase-associated with diverse cellular activities (AAA+-class, ATPase) with a winged-helix DNA-binding domain. The RuvB-ADP complex, structure and mutagenesis suggest how AAA+-class ATPases couple nucleotide, binding and hydrolysis to interdomain conformational changes and asymmetry, within the RuvB hexamer implied by the crystallographic packing and, small-angle X-ray scattering in solution. ATP-driven domain motion is, positioned to move double-stranded DNA through the hexamer and drive, conformational changes between subunits by altering the complementary, hydrophilic protein- protein interfaces. Structural and biochemical, analysis of five motifs in the protein suggest that ATP binding is a, strained conformation recognized both by sensors and the Walker motifs and, that intersubunit activation occurs by an arginine finger motif, reminiscent of the GTPase-activating proteins. Taken together, these, results provide insights into how RuvB functions as a motor for branch, migration of Holliday junctions.
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<StructureSection load='1in8' size='340' side='right'caption='[[1in8]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1in8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IN8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IN8 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1in8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1in8 OCA], [https://pdbe.org/1in8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1in8 RCSB], [https://www.ebi.ac.uk/pdbsum/1in8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1in8 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RUVB_THEMA RUVB_THEMA] The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/in/1in8_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1in8 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The RuvB hexamer is the chemomechanical motor of the RuvAB complex that migrates Holliday junction branch-points in DNA recombination and the rescue of stalled DNA replication forks. The 1.6 A crystal structure of Thermotoga maritima RuvB together with five mutant structures reveal that RuvB is an ATPase-associated with diverse cellular activities (AAA+-class ATPase) with a winged-helix DNA-binding domain. The RuvB-ADP complex structure and mutagenesis suggest how AAA+-class ATPases couple nucleotide binding and hydrolysis to interdomain conformational changes and asymmetry within the RuvB hexamer implied by the crystallographic packing and small-angle X-ray scattering in solution. ATP-driven domain motion is positioned to move double-stranded DNA through the hexamer and drive conformational changes between subunits by altering the complementary hydrophilic protein- protein interfaces. Structural and biochemical analysis of five motifs in the protein suggest that ATP binding is a strained conformation recognized both by sensors and the Walker motifs and that intersubunit activation occurs by an arginine finger motif reminiscent of the GTPase-activating proteins. Taken together, these results provide insights into how RuvB functions as a motor for branch migration of Holliday junctions.
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==About this Structure==
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Structure and mechanism of the RuvB Holliday junction branch migration motor.,Putnam CD, Clancy SB, Tsuruta H, Gonzalez S, Wetmur JG, Tainer JA J Mol Biol. 2001 Aug 10;311(2):297-310. PMID:11478862<ref>PMID:11478862</ref>
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1IN8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IN8 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure and mechanism of the RuvB Holliday junction branch migration motor., Putnam CD, Clancy SB, Tsuruta H, Gonzalez S, Wetmur JG, Tainer JA, J Mol Biol. 2001 Aug 10;311(2):297-310. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11478862 11478862]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1in8" style="background-color:#fffaf0;"></div>
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[[Category: Thermotoga maritima]]
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[[Category: Clancy, S.B.]]
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[[Category: Putnam, C.D.]]
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[[Category: Tainer, J.A.]]
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[[Category: Tsuruta, H.]]
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[[Category: Wetmur, J.G.]]
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[[Category: ADP]]
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[[Category: aaa+-class atpase]]
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[[Category: arginine finger]]
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[[Category: branch migration motor]]
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[[Category: dna translocase]]
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[[Category: holliday junction]]
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[[Category: sensor 1]]
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[[Category: sensor 2]]
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[[Category: walker a]]
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[[Category: walker b]]
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[[Category: winged-helix domain]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:31:07 2007''
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==See Also==
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermotoga maritima]]
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[[Category: Clancy SB]]
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[[Category: Putnam CD]]
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[[Category: Tainer JA]]
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[[Category: Tsuruta H]]
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[[Category: Wetmur JG]]

Current revision

THERMOTOGA MARITIMA RUVB T158V

PDB ID 1in8

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