3ifb

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{{Seed}}
 
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[[Image:3ifb.png|left|200px]]
 
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==NMR STUDY OF HUMAN INTESTINAL FATTY ACID BINDING PROTEIN==
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The line below this paragraph, containing "STRUCTURE_3ifb", creates the "Structure Box" on the page.
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<StructureSection load='3ifb' size='340' side='right'caption='[[3ifb]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ifb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IFB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IFB FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ifb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ifb OCA], [https://pdbe.org/3ifb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ifb RCSB], [https://www.ebi.ac.uk/pdbsum/3ifb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ifb ProSAT]</span></td></tr>
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{{STRUCTURE_3ifb| PDB=3ifb | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/FABPI_HUMAN FABPI_HUMAN] FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/if/3ifb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ifb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The human intestinal fatty acid binding protein (I-FABP) is a small (131 amino acids) protein which binds dietary long-chain fatty acids in the cytosol of enterocytes. Recently, an alanine to threonine substitution at position 54 in I-FABP has been identified which affects fatty acid binding and transport, and is associated with the development of insulin resistance in several populations including Mexican-Americans and Pima Indians. To investigate the molecular basis of the binding properties of I-FABP, the 3D solution structure of the more common form of human I-FABP (Ala54) was studied by multidimensional NMR spectroscopy. Recombinant I-FABP was expressed from E. coli in the presence and absence of 15N-enriched media. The sequential assignments for non-delipidated I-FABP were completed by using 2D homonuclear spectra (COSY, TOCSY and NOESY) and 3D heteronuclear spectra (NOESY-HMQC and TOCSY-HMQC). The tertiary structure of human I-FABP was calculated by using the distance geometry program DIANA based on 2519 distance constraints obtained from the NMR data. Subsequent energy minimization was carried out by using the program SYBYL in the presence of distance constraints. The conformation of human I-FABP consists of 10 antiparallel beta-strands which form two nearly orthogonal beta-sheets of five strands each, and two short alpha-helices that connect the beta-strands A and B. The interior of the protein consists of a water-filled cavity between the two beta-sheets. The NMR solution structure of human I-FABP is similar to the crystal structure of rat I-FABP. The NMR results show significant conformational variability of certain backbone segments around the postulated portal region for the entry and exit of fatty acid ligand.
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===NMR STUDY OF HUMAN INTESTINAL FATTY ACID BINDING PROTEIN===
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Solution structure of human intestinal fatty acid binding protein: implications for ligand entry and exit.,Zhang F, Lucke C, Baier LJ, Sacchettini JC, Hamilton JA J Biomol NMR. 1997 Apr;9(3):213-28. PMID:9204553<ref>PMID:9204553</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ifb" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_9204553}}, adds the Publication Abstract to the page
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*[[Fatty acid-binding protein 3D structures|Fatty acid-binding protein 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 9204553 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9204553}}
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__TOC__
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</StructureSection>
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==About this Structure==
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3IFB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IFB OCA].
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==Reference==
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Solution structure of human intestinal fatty acid binding protein: implications for ligand entry and exit., Zhang F, Lucke C, Baier LJ, Sacchettini JC, Hamilton JA, J Biomol NMR. 1997 Apr;9(3):213-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9204553 9204553]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Baier, L J.]]
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[[Category: Baier LJ]]
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[[Category: Hamilton, J A.]]
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[[Category: Hamilton JA]]
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[[Category: Luecke, C.]]
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[[Category: Luecke C]]
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[[Category: Sacchettini, J C.]]
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[[Category: Sacchettini JC]]
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[[Category: Zhang, F.]]
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[[Category: Zhang F]]
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[[Category: Fatty acid binding]]
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[[Category: Fatty acid binding protein]]
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[[Category: Intracellular lipid binding protein]]
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[[Category: Single base polymorphism]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 12:50:52 2008''
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Current revision

NMR STUDY OF HUMAN INTESTINAL FATTY ACID BINDING PROTEIN

PDB ID 3ifb

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