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1iro

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(New page: 200px<br /><applet load="1iro" size="450" color="white" frame="true" align="right" spinBox="true" caption="1iro, resolution 1.1&Aring;" /> '''RUBREDOXIN (OXIDIZED,...)
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[[Image:1iro.gif|left|200px]]<br /><applet load="1iro" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1iro, resolution 1.1&Aring;" />
 
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'''RUBREDOXIN (OXIDIZED, FE(III)) AT 1.1 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==RUBREDOXIN (OXIDIZED, FE(III)) AT 1.1 ANGSTROMS RESOLUTION==
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The Zn(Scys)4 unit is present in numerous proteins, where it assumes, structural, regulatory, or catalytic roles. The same coordination is found, naturally around iron in rubredoxins, several structures of which have, been refined at resolutions of, or near to, 1 A. The fold of the small, protein rubredoxin around its metal ion is an excellent model for many, zinc finger proteins. Zn-substituted rubredoxin and its Fe-containing, counterpart were both obtained as the products of the expression in, Escherichia coli of the rubredoxin-encoding gene from Clostridium, pasteurianum. The structures of both proteins have been refined with an, anisotropic model at atomic resolution (1.1 A, R = 8.3% for Fe-rubredoxin, and 1.2 A, R = 9.6% for Zn-rubredoxin) and are very similar. The most, significant differences are increased lengths of the M-S bonds in, Zn-rubredoxin (average length, 2.345 A) as compared with Fe-rubredoxin, (average length, 2.262 A). An increase of the CA-CB-SG-M dihedral angles, involving Cys-6 and Cys-39, the first cysteines of each of the, Cys-Xaa-Xaa-Cys metal binding motifs, has been observed. Another, consequence of the replacement of iron by zinc is that the region around, residues 36-46 undergoes larger displacements than the remainder of the, polypeptide chain. Despite these changes, the main features of the FeS4, site, namely a local 2-fold symmetry and the characteristic network of, N-H...S hydrogen bonds, are conserved in the ZnS4 site. The Zn-substituted, rubredoxin provides the first precise structure of a Zn(Scys)4 unit in a, protein. The nearly identical fold of rubredoxin around iron or zinc, suggests that at least in some of the sites where the metal has mainly a, structural role-e.g., zinc fingers-the choice of the relevant metal may be, directed by its cellular availability and mobilization processes rather, than by its chemical nature.
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<StructureSection load='1iro' size='340' side='right'caption='[[1iro]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1iro]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IRO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IRO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1iro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1iro OCA], [https://pdbe.org/1iro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1iro RCSB], [https://www.ebi.ac.uk/pdbsum/1iro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1iro ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RUBR_CLOPA RUBR_CLOPA] Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ir/1iro_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1iro ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1IRO is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_pasteurianum Clostridium pasteurianum] with FE as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IRO OCA].
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*[[Rubredoxin|Rubredoxin]]
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*[[Rubredoxin 3D structures|Rubredoxin 3D structures]]
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==Reference==
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*[[Rubredoxin PDB structures|Rubredoxin PDB structures]]
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Zinc- and iron-rubredoxins from Clostridium pasteurianum at atomic resolution: a high-precision model of a ZnS4 coordination unit in a protein., Dauter Z, Wilson KS, Sieker LC, Moulis JM, Meyer J, Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8836-40. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8799113 8799113]
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__TOC__
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</StructureSection>
[[Category: Clostridium pasteurianum]]
[[Category: Clostridium pasteurianum]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Dauter, Z.]]
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[[Category: Dauter Z]]
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[[Category: Meyer, J.]]
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[[Category: Meyer J]]
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[[Category: Moulis, J.M.]]
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[[Category: Moulis JM]]
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[[Category: Sieker, L.C.]]
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[[Category: Sieker LC]]
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[[Category: Wilson, K.S.]]
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[[Category: Wilson KS]]
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[[Category: FE]]
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[[Category: electron transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:36:45 2007''
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Current revision

RUBREDOXIN (OXIDIZED, FE(III)) AT 1.1 ANGSTROMS RESOLUTION

PDB ID 1iro

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