3ygs

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{{Seed}}
 
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[[Image:3ygs.png|left|200px]]
 
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==APAF-1 CARD IN COMPLEX WITH PRODOMAIN OF PROCASPASE-9==
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The line below this paragraph, containing "STRUCTURE_3ygs", creates the "Structure Box" on the page.
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<StructureSection load='3ygs' size='340' side='right'caption='[[3ygs]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3ygs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3YGS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3YGS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ygs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ygs OCA], [https://pdbe.org/3ygs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ygs RCSB], [https://www.ebi.ac.uk/pdbsum/3ygs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ygs ProSAT]</span></td></tr>
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{{STRUCTURE_3ygs| PDB=3ygs | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/APAF_HUMAN APAF_HUMAN] Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.<ref>PMID:10393175</ref> <ref>PMID:12804598</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yg/3ygs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ygs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Caspase-9-mediated apoptosis (programmed cell death) plays a central role in the development and homeostasis of all multicellular organisms. Mature caspase-9 is derived from its procaspase precursor as a result of recruitment by the activating factor Apaf-1. The crystal structures of the caspase-recruitment domain of Apaf-1 by itself and in complex with the prodomain of procaspase-9 have been determined at 1.6 and 2.5 A resolution, respectively. These structures and other evidence reveal that each molecule of Apaf-1 interacts with a molecule of procaspase-9 through two highly charged and complementary surfaces formed by non-conserved residues; these surfaces determine recognition specificity through networks of intermolecular hydrogen bonds and van der Waals interactions. Mutation of the important interface residues in procaspase-9 or Apaf-1 prevents or reduces activation of procaspase-9 in a cell-free system. Wild-type, but not mutant, prodomains of caspase-9 completely inhibit catalytic processing of procaspase-9. Furthermore, analysis of homologues from Caenorhabditis elegans indicates that recruitment of CED-3 by CED-4 is probably mediated by the same set of conserved structural motifs, with a corresponding change in the specificity-determining residues.
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===APAF-1 CARD IN COMPLEX WITH PRODOMAIN OF PROCASPASE-9===
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Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1.,Qin H, Srinivasula SM, Wu G, Fernandes-Alnemri T, Alnemri ES, Shi Y Nature. 1999 Jun 10;399(6736):549-57. PMID:10376594<ref>PMID:10376594</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3ygs" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_10376594}}, adds the Publication Abstract to the page
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*[[Apoptotic protease-activating factor-1 3D structures|Apoptotic protease-activating factor-1 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 10376594 is the PubMed ID number.
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*[[Caspase 3D structures|Caspase 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_10376594}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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3YGS is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3YGS OCA].
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==Reference==
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Structural basis of procaspase-9 recruitment by the apoptotic protease-activating factor 1., Qin H, Srinivasula SM, Wu G, Fernandes-Alnemri T, Alnemri ES, Shi Y, Nature. 1999 Jun 10;399(6736):549-57. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10376594 10376594]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Alnemri, E.]]
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[[Category: Alnemri E]]
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[[Category: Fernandes-Alnemri, T.]]
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[[Category: Fernandes-Alnemri T]]
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[[Category: Qin, H.]]
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[[Category: Qin H]]
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[[Category: Shi, Y.]]
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[[Category: Shi Y]]
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[[Category: Srinivasula, S.]]
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[[Category: Srinivasula S]]
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[[Category: Wu, G.]]
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[[Category: Wu G]]
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[[Category: Apoptosis]]
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[[Category: Caspase activation]]
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[[Category: Caspase recruitment]]
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[[Category: Recognition complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:13:30 2008''
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Current revision

APAF-1 CARD IN COMPLEX WITH PRODOMAIN OF PROCASPASE-9

PDB ID 3ygs

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