1ivv

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="1ivv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ivv, resolution 2.1&Aring;" /> '''Crystal structure of ...)
Current revision (06:47, 30 October 2024) (edit) (undo)
 
(16 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1ivv.jpg|left|200px]]<br /><applet load="1ivv" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1ivv, resolution 2.1&Aring;" />
 
-
'''Crystal structure of copper amine oxidase from Arthrobacter globiformis: Early intermediate in topaquinone biogenesis'''<br />
 
-
==Overview==
+
==Crystal structure of copper amine oxidase from Arthrobacter globiformis: Early intermediate in topaquinone biogenesis==
-
The quinone cofactor TPQ in copper amine oxidase is generated by, posttranslational modification of an active site tyrosine residue. Using, X-ray crystallography, we have probed the copper-dependent autooxidation, process of TPQ in the enzyme from Arthrobacter globiformis. Apo enzyme, crystals were anaerobically soaked with copper; the structure determined, from this crystal provides a view of the initial state: the unmodified, tyrosine coordinated to the bound copper. Exposure of the copper-bound, crystals to oxygen led to the formation of freeze-trapped intermediates;, structural analyses indicate that these intermediates contain, dihydroxyphenylalanine quinone and trihydroxyphenylalanine. These are the, first visualized intermediates during TPQ biogenesis in copper amine, oxidase.
+
<StructureSection load='1ivv' size='340' side='right'caption='[[1ivv]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1ivv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IVV FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=DAH:3,4-DIHYDROXYPHENYLALANINE'>DAH</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ivv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ivv OCA], [https://pdbe.org/1ivv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ivv RCSB], [https://www.ebi.ac.uk/pdbsum/1ivv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ivv ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PAOX_ARTGO PAOX_ARTGO]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/iv/1ivv_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ivv ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The quinone cofactor TPQ in copper amine oxidase is generated by posttranslational modification of an active site tyrosine residue. Using X-ray crystallography, we have probed the copper-dependent autooxidation process of TPQ in the enzyme from Arthrobacter globiformis. Apo enzyme crystals were anaerobically soaked with copper; the structure determined from this crystal provides a view of the initial state: the unmodified tyrosine coordinated to the bound copper. Exposure of the copper-bound crystals to oxygen led to the formation of freeze-trapped intermediates; structural analyses indicate that these intermediates contain dihydroxyphenylalanine quinone and trihydroxyphenylalanine. These are the first visualized intermediates during TPQ biogenesis in copper amine oxidase.
-
==About this Structure==
+
X-ray snapshots of quinone cofactor biogenesis in bacterial copper amine oxidase.,Kim M, Okajima T, Kishishita S, Yoshimura M, Kawamori A, Tanizawa K, Yamaguchi H Nat Struct Biol. 2002 Aug;9(8):591-6. PMID:12134140<ref>PMID:12134140</ref>
-
1IVV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis] with CU as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Amine_oxidase_(copper-containing) Amine oxidase (copper-containing)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.4.3.6 1.4.3.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IVV OCA].
+
-
==Reference==
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
X-ray snapshots of quinone cofactor biogenesis in bacterial copper amine oxidase., Kim M, Okajima T, Kishishita S, Yoshimura M, Kawamori A, Tanizawa K, Yamaguchi H, Nat Struct Biol. 2002 Aug;9(8):591-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12134140 12134140]
+
</div>
-
[[Category: Amine oxidase (copper-containing)]]
+
<div class="pdbe-citations 1ivv" style="background-color:#fffaf0;"></div>
-
[[Category: Arthrobacter globiformis]]
+
-
[[Category: Single protein]]
+
-
[[Category: Kawamori, A.]]
+
-
[[Category: Kim, M.]]
+
-
[[Category: Kishishita, S.]]
+
-
[[Category: Okajima, T.]]
+
-
[[Category: Tanizawa, K.]]
+
-
[[Category: Yamaguchi, H.]]
+
-
[[Category: Yoshimura, M.]]
+
-
[[Category: CU]]
+
-
[[Category: amine oxidase]]
+
-
[[Category: biogenesis]]
+
-
[[Category: copper]]
+
-
[[Category: dah]]
+
-
[[Category: freeze-trapp]]
+
-
[[Category: intermediate]]
+
-
[[Category: oxidoreductase]]
+
-
[[Category: quinone cofactor]]
+
-
[[Category: tpq]]
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:42:42 2007''
+
==See Also==
 +
*[[Copper amine oxidase 3D structures|Copper amine oxidase 3D structures]]
 +
== References ==
 +
<references/>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Arthrobacter globiformis]]
 +
[[Category: Large Structures]]
 +
[[Category: Kawamori A]]
 +
[[Category: Kim M]]
 +
[[Category: Kishishita S]]
 +
[[Category: Okajima T]]
 +
[[Category: Tanizawa K]]
 +
[[Category: Yamaguchi H]]
 +
[[Category: Yoshimura M]]

Current revision

Crystal structure of copper amine oxidase from Arthrobacter globiformis: Early intermediate in topaquinone biogenesis

PDB ID 1ivv

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ivv"
Personal tools