4pga

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{{Seed}}
 
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[[Image:4pga.png|left|200px]]
 
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==GLUTAMINASE-ASPARAGINASE FROM PSEUDOMONAS 7A==
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The line below this paragraph, containing "STRUCTURE_4pga", creates the "Structure Box" on the page.
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<StructureSection load='4pga' size='340' side='right'caption='[[4pga]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4pga]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._7A Pseudomonas sp. 7A]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PGA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PGA FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_4pga| PDB=4pga | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pga FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pga OCA], [https://pdbe.org/4pga PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pga RCSB], [https://www.ebi.ac.uk/pdbsum/4pga PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pga ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ASPQ_PSES7 ASPQ_PSES7]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pg/4pga_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4pga ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Pseudomonas 7A glutaminase-asparaginase (PGA) catalyzes the hydrolysis of D- and L-isomers of glutamine and asparagine. X-ray quality type-1 crystals of PGA have been obtained from 2.0 M ammonium sulfate. The space group is C222(1) with unit-cell dimensions a = 78.62, b = 135.80, and c = 137.88 A. The tetrameric molecule is located on a crystallographic 2-fold axis, and two subunits form the asymmetric portion of the unit cell. The structure was solved by the molecular replacement method and refined at 1.7 A resolution to an R = 19.9% with a good geometry of the model, G = 0.05. The resultant electron density maps enabled us to resolve individual constituent atoms of most residues and introduce minor revisions to the amino acid sequence. The catalytic loop, Thr20-Gly40, is in the closed conformation with excellent electron density in both subunits. A sulfate ion and an ammonium ion are bound in the substrate binding site and interect with the loop. This interaction appears to be responsible for the observed closed conformation. New arguments supporting Thr20 as the catalytic nucleophile in the asparaginase activity are proposed.
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===GLUTAMINASE-ASPARAGINASE FROM PSEUDOMONAS 7A===
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Ion binding induces closed conformation in Pseudomonas 7A glutaminase-asparaginase (PGA): crystal structure of the PGA-SO4(2-)-NH4+ complex at 1.7 A resolution.,Jakob CG, Lewinski K, LaCount MW, Roberts J, Lebioda L Biochemistry. 1997 Jan 28;36(4):923-31. PMID:9020792<ref>PMID:9020792</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4pga" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_9020792}}, adds the Publication Abstract to the page
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*[[Glutaminase 3D structures|Glutaminase 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 9020792 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_9020792}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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4PGA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_sp._7a Pseudomonas sp. 7a]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PGA OCA].
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[[Category: Pseudomonas sp. 7A]]
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[[Category: Jakob CG]]
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==Reference==
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[[Category: Lacount MW]]
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Ion binding induces closed conformation in Pseudomonas 7A glutaminase-asparaginase (PGA): crystal structure of the PGA-SO4(2-)-NH4+ complex at 1.7 A resolution., Jakob CG, Lewinski K, LaCount MW, Roberts J, Lebioda L, Biochemistry. 1997 Jan 28;36(4):923-31. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9020792 9020792]
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[[Category: Lebioda L]]
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[[Category: Pseudomonas sp. 7a]]
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[[Category: Lewinski K]]
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[[Category: Single protein]]
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[[Category: Roberts J]]
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[[Category: Jakob, C G.]]
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[[Category: Lacount, M W.]]
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[[Category: Lebioda, L.]]
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[[Category: Lewinski, K.]]
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[[Category: Roberts, J.]]
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[[Category: Bacterial amidohydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:47:10 2008''
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Current revision

GLUTAMINASE-ASPARAGINASE FROM PSEUDOMONAS 7A

PDB ID 4pga

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