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4vub

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{{Seed}}
 
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[[Image:4vub.png|left|200px]]
 
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<!--
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==CCDB, A TOPOISOMERASE POISON FROM ESCHERICHIA COLI==
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The line below this paragraph, containing "STRUCTURE_4vub", creates the "Structure Box" on the page.
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<StructureSection load='4vub' size='340' side='right'caption='[[4vub]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[4vub]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4VUB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4VUB FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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{{STRUCTURE_4vub| PDB=4vub | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4vub FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4vub OCA], [https://pdbe.org/4vub PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4vub RCSB], [https://www.ebi.ac.uk/pdbsum/4vub PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4vub ProSAT]</span></td></tr>
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</table>
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===CCDB, A TOPOISOMERASE POISON FROM ESCHERICHIA COLI===
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== Function ==
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[https://www.uniprot.org/uniprot/CCDB_ECOLI CCDB_ECOLI] Toxic component of a toxin-antitoxin (TA) module, functioning in plasmid maintainence. Responsible for the post-segregational killing (PSK) of plasmid-free cells, also referred to as a plasmid addiction system. Half-life of over 2 hours. Cell killing by CcdB is accompanied by filamentation, defects in chromosome and plasmid segregation, defects in cell division, formation of anucleate cells, decreased DNA synthesis and plasmid loss. Interferes with the activity of DNA gyrase, inducing it to form a covalent GyrA-DNA complex that cannot be resolved, thus promoting breakage of plasmid and chromosomal DNA. DNA breakage requires hydrolyzable ATP. Toxicity is inhibited by labile antitoxin CcdA, which blocks the activity of CcdB; CcdA also removes bound CcdB protein from the CcdB-GyrA complex by forming a CcdA-CcdB complex, a process termed rejuvenation. Also acts to inhibit partitioning of the chromosomal DNA. Functions as a transcriptional corepressor for the ccdAB operon, repression also requires CcdA.<ref>PMID:6327993</ref> <ref>PMID:2651399</ref> <ref>PMID:6308648</ref> <ref>PMID:2615761</ref> <ref>PMID:1324324</ref> <ref>PMID:8604132</ref>
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== Evolutionary Conservation ==
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<!--
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[[Image:Consurf_key_small.gif|200px|right]]
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The line below this paragraph, {{ABSTRACT_PUBMED_9917404}}, adds the Publication Abstract to the page
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Check<jmol>
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(as it appears on PubMed at http://www.pubmed.gov), where 9917404 is the PubMed ID number.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vu/4vub_consurf.spt"</scriptWhenChecked>
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{{ABSTRACT_PUBMED_9917404}}
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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4VUB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4VUB OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=4vub ConSurf].
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<div style="clear:both"></div>
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==Reference==
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== References ==
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Crystal structure of CcdB, a topoisomerase poison from E. coli., Loris R, Dao-Thi MH, Bahassi EM, Van Melderen L, Poortmans F, Liddington R, Couturier M, Wyns L, J Mol Biol. 1999 Jan 29;285(4):1667-77. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9917404 9917404]
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bahasi, E M.]]
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[[Category: Bahasi EM]]
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[[Category: Couturier, M.]]
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[[Category: Couturier M]]
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[[Category: Dao-Thi, M H.]]
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[[Category: Dao-Thi M-H]]
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[[Category: Liddington, R.]]
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[[Category: Liddington R]]
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[[Category: Loris, R.]]
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[[Category: Loris R]]
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[[Category: Melderen, L Van.]]
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[[Category: Poortmans F]]
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[[Category: Poortmans, F.]]
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[[Category: Van Melderen L]]
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[[Category: Wyns, L.]]
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[[Category: Wyns L]]
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[[Category: Ccdb]]
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[[Category: Plasmid]]
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[[Category: Plasmid addiction]]
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[[Category: Topoisomerase poison]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 13:54:26 2008''
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Current revision

CCDB, A TOPOISOMERASE POISON FROM ESCHERICHIA COLI

PDB ID 4vub

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