1ixs

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(New page: 200px<br /><applet load="1ixs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ixs, resolution 3.2&Aring;" /> '''Structure of RuvB com...)
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[[Image:1ixs.gif|left|200px]]<br /><applet load="1ixs" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ixs, resolution 3.2&Aring;" />
 
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'''Structure of RuvB complexed with RuvA domain III'''<br />
 
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==Overview==
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==Structure of RuvB complexed with RuvA domain III==
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We present the X-ray structure of the RuvA-RuvB complex, which plays a, crucial role in ATP-dependent branch migration. Two RuvA tetramers form, the symmetric and closed octameric shell, where four RuvA domain IIIs, spring out in the two opposite directions to be individually caught by a, single RuvB. The binding of domain III deforms the protruding beta hairpin, in the N-terminal domain of RuvB and thereby appears to induce a, functional and less symmetric RuvB hexameric ring. The model of the, RuvA-RuvB junction DNA ternary complex, constructed by fitting the X-ray, structure into the averaged electron microscopic images of the RuvA-RuvB, junction, appears to be more compatible with the branch migration mode of, a fixed RuvA-RuvB interaction than with a rotational interaction mode.
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<StructureSection load='1ixs' size='340' side='right'caption='[[1ixs]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ixs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IXS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ixs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ixs OCA], [https://pdbe.org/1ixs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ixs RCSB], [https://www.ebi.ac.uk/pdbsum/1ixs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ixs ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RUVA_THET8 RUVA_THET8] The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ix/1ixs_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ixs ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We present the X-ray structure of the RuvA-RuvB complex, which plays a crucial role in ATP-dependent branch migration. Two RuvA tetramers form the symmetric and closed octameric shell, where four RuvA domain IIIs spring out in the two opposite directions to be individually caught by a single RuvB. The binding of domain III deforms the protruding beta hairpin in the N-terminal domain of RuvB and thereby appears to induce a functional and less symmetric RuvB hexameric ring. The model of the RuvA-RuvB junction DNA ternary complex, constructed by fitting the X-ray structure into the averaged electron microscopic images of the RuvA-RuvB junction, appears to be more compatible with the branch migration mode of a fixed RuvA-RuvB interaction than with a rotational interaction mode.
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==About this Structure==
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Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery.,Yamada K, Miyata T, Tsuchiya D, Oyama T, Fujiwara Y, Ohnishi T, Iwasaki H, Shinagawa H, Ariyoshi M, Mayanagi K, Morikawa K Mol Cell. 2002 Sep;10(3):671-81. PMID:12408833<ref>PMID:12408833</ref>
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1IXS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus] with ANP as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Adenosinetriphosphatase Adenosinetriphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.3 3.6.1.3] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1IXS OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Crystal structure of the RuvA-RuvB complex: a structural basis for the Holliday junction migrating motor machinery., Yamada K, Miyata T, Tsuchiya D, Oyama T, Fujiwara Y, Ohnishi T, Iwasaki H, Shinagawa H, Ariyoshi M, Mayanagi K, Morikawa K, Mol Cell. 2002 Sep;10(3):671-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12408833 12408833]
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</div>
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[[Category: Adenosinetriphosphatase]]
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<div class="pdbe-citations 1ixs" style="background-color:#fffaf0;"></div>
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[[Category: Protein complex]]
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[[Category: Thermus thermophilus]]
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[[Category: Ariyoshi, M.]]
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[[Category: Fujiwara, Y.]]
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[[Category: Iwasaki, H.]]
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[[Category: Mayanagi, K.]]
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[[Category: Miyata, T.]]
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[[Category: Morikawa, K.]]
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[[Category: Ohnishi, T.]]
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[[Category: Oyama, T.]]
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[[Category: Shinagawa, H.]]
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[[Category: Tsuchiya, D.]]
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[[Category: Yamada, K.]]
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[[Category: ANP]]
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[[Category: aaa-atpase domain]]
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[[Category: complex with nucleotide]]
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[[Category: heterodimeric protein complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 17:45:31 2007''
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==See Also==
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*[[Helicase 3D structures|Helicase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Thermus thermophilus]]
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[[Category: Ariyoshi M]]
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[[Category: Fujiwara Y]]
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[[Category: Iwasaki H]]
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[[Category: Mayanagi K]]
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[[Category: Miyata T]]
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[[Category: Morikawa K]]
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[[Category: Ohnishi T]]
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[[Category: Oyama T]]
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[[Category: Shinagawa H]]
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[[Category: Tsuchiya D]]
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[[Category: Yamada K]]

Current revision

Structure of RuvB complexed with RuvA domain III

PDB ID 1ixs

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