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1bvv

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(New page: 200px<br /> <applet load="1bvv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bvv, resolution 1.8&Aring;" /> '''SUGAR RING DISTORTIO...)
Current revision (15:36, 13 March 2024) (edit) (undo)
 
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[[Image:1bvv.gif|left|200px]]<br />
 
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<applet load="1bvv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bvv, resolution 1.8&Aring;" />
 
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'''SUGAR RING DISTORTION IN THE GLYCOSYL-ENZYME INTERMEDIATE OF A FAMILY G/11 XYLANASE'''<br />
 
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==Overview==
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==SUGAR RING DISTORTION IN THE GLYCOSYL-ENZYME INTERMEDIATE OF A FAMILY G/11 XYLANASE==
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The 1.8 A resolution structure of the glycosyl-enzyme intermediate formed, on the retaining beta-1,4-xylanase from Bacillus circulans has been, determined using X-ray crystallographic techniques. The 2-fluoro-xylose, residue bound in the -1 subsite adopts a 2,5B (boat) conformation, allowing atoms C5, O5, C1, and C2 of the sugar to achieve coplanarity as, required at the oxocarbenium ion-like transition states of the, double-displacement catalytic mechanism. Comparison of this structure to, that of a mutant of this same enzyme noncovalently complexed with, xylotetraose [Wakarchuk et al. (1994) Protein Sci. 3, 467-475] reveals a, number of differences beyond the distortion of the sugar moiety. Most, notably, a bifurcated hydrogen bond interaction is formed in the, glycosyl-enzyme ... [[http://ispc.weizmann.ac.il/pmbin/getpm?10220321 (full description)]]
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<StructureSection load='1bvv' size='340' side='right'caption='[[1bvv]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1bvv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Niallia_circulans Niallia circulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BVV FirstGlance]. <br>
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1BVV is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Bacillus_circulans Bacillus circulans]] with XYP and DFX as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BVV OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DFX:1,2-DEOXY-2-FLUORO-XYLOPYRANOSE'>DFX</scene>, <scene name='pdbligand=XYP:BETA-D-XYLOPYRANOSE'>XYP</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bvv OCA], [https://pdbe.org/1bvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bvv RCSB], [https://www.ebi.ac.uk/pdbsum/1bvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bvv ProSAT]</span></td></tr>
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Sugar ring distortion in the glycosyl-enzyme intermediate of a family G/11 xylanase., Sidhu G, Withers SG, Nguyen NT, McIntosh LP, Ziser L, Brayer GD, Biochemistry. 1999 Apr 27;38(17):5346-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10220321 10220321]
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</table>
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[[Category: Bacillus circulans]]
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== Function ==
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[[Category: Single protein]]
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[https://www.uniprot.org/uniprot/XYNA_NIACI XYNA_NIACI]
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[[Category: Brayer, G.D.]]
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== Evolutionary Conservation ==
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[[Category: Sidhu, G.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: DFX]]
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Check<jmol>
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[[Category: XYP]]
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<jmolCheckbox>
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[[Category: boat conformation]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bv/1bvv_consurf.spt"</scriptWhenChecked>
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[[Category: glycosidase]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: glycosyl-enzyme intermediate]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: hydrolase]]
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</jmolCheckbox>
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[[Category: xylan degradation]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bvv ConSurf].
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[[Category: xylanase]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 20:05:37 2007''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Niallia circulans]]
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[[Category: Brayer GD]]
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[[Category: Sidhu G]]

Current revision

SUGAR RING DISTORTION IN THE GLYCOSYL-ENZYME INTERMEDIATE OF A FAMILY G/11 XYLANASE

PDB ID 1bvv

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