2vos

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{{Seed}}
 
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[[Image:2vos.png|left|200px]]
 
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==Mycobacterium tuberculosis Folylpolyglutamate synthase complexed with ADP==
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The line below this paragraph, containing "STRUCTURE_2vos", creates the "Structure Box" on the page.
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<StructureSection load='2vos' size='340' side='right'caption='[[2vos]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2vos]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VOS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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{{STRUCTURE_2vos| PDB=2vos | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vos OCA], [https://pdbe.org/2vos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vos RCSB], [https://www.ebi.ac.uk/pdbsum/2vos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vos ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/O53174_MYCTO O53174_MYCTO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vo/2vos_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vos ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Folate derivatives are essential vitamins for cell growth and replication, primarily because of their central role in reactions of one-carbon metabolism. Folates require polyglutamation to be efficiently retained within the cell and folate-dependent enzymes have a higher affinity for the polyglutamylated forms of this cofactor. Polyglutamylation is dependent on the enzyme folylpolyglutamate synthetase (FPGS), which catalyzes the sequential addition of several glutamates to folate. FPGS is essential for the growth and survival of important bacterial species, including Mycobacterium tuberculosis, and is a potential drug target. Here, the crystal structures of M. tuberculosis FPGS in complex with ADP and AMPPCP are reported at 2.0 and 2.3 angstroms resolution, respectively. The structures reveal a deeply buried nucleotide-binding site, as in the Escherichia coli and Lactobacillus casei FPGS structures, and a long extended groove for the binding of folate substrates. Differences from the E. coli and L. casei FPGS structures are seen in the binding of a key divalent cation, the carbamylation state of an essential lysine side chain and the adoption of an 'open' position by the active-site beta5-alpha6 loop. These changes point to coordinated events that are associated with dihydropteroate/folate binding and the catalysis of the new amide bond with an incoming glutamate residue.
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===MYCOBACTERIUM TUBERCULOSIS FOLYLPOLYGLUTAMATE SYNTHASE COMPLEXED WITH ADP===
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Structures of Mycobacterium tuberculosis folylpolyglutamate synthase complexed with ADP and AMPPCP.,Young PG, Smith CA, Metcalf P, Baker EN Acta Crystallogr D Biol Crystallogr. 2008 Jul;D64(Pt 7):745-53. Epub 2008, Jun 18. PMID:18566510<ref>PMID:18566510</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2vos" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18566510}}, adds the Publication Abstract to the page
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*[[Folylpolyglutamate synthase|Folylpolyglutamate synthase]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18566510 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18566510}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2VOS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VOS OCA].
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[[Category: Mycobacterium tuberculosis H37Rv]]
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[[Category: Baker EN]]
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==Reference==
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[[Category: Metcalf P]]
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Structures of Mycobacterium tuberculosisfolylpolyglutamate synthase complexed with ADP and AMPPCP., Young PG, Smith CA, Metcalf P, Baker EN, Acta Crystallogr D Biol Crystallogr. 2008 Jul;64(Pt 7):745-53. Epub 2008, Jun 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18566510 18566510]
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[[Category: Smith CA]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Young PG]]
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[[Category: Single protein]]
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[[Category: Tetrahydrofolate synthase]]
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[[Category: Baker, E N.]]
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[[Category: Metcalf, P.]]
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[[Category: Smith, C A.]]
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[[Category: Young, P G.]]
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[[Category: Atp-binding]]
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[[Category: Cell cycle]]
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[[Category: Cell division]]
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[[Category: Cell shape]]
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[[Category: Cell wall biogenesis/degradation]]
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[[Category: Cobalt binding]]
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[[Category: Conformational change]]
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[[Category: Folate metabolism]]
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[[Category: Ligase]]
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[[Category: Nucleotide binding]]
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[[Category: Nucleotide-binding]]
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[[Category: Peptidoglycan synthesis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 10 13:22:36 2008''
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Current revision

Mycobacterium tuberculosis Folylpolyglutamate synthase complexed with ADP

PDB ID 2vos

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