2qhr

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{{Seed}}
 
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[[Image:2qhr.png|left|200px]]
 
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==Crystal structure of the 13F6-1-2 Fab fragment bound to its Ebola virus glycoprotein peptide epitope.==
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The line below this paragraph, containing "STRUCTURE_2qhr", creates the "Structure Box" on the page.
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<StructureSection load='2qhr' size='340' side='right'caption='[[2qhr]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2qhr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus_-_Eckron_(Zaire,_1976) Ebola virus - Eckron (Zaire, 1976)] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QHR FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qhr OCA], [https://pdbe.org/2qhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qhr RCSB], [https://www.ebi.ac.uk/pdbsum/2qhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qhr ProSAT]</span></td></tr>
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{{STRUCTURE_2qhr| PDB=2qhr | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LAC2_MOUSE LAC2_MOUSE]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qh/2qhr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qhr ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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13F6-1-2 is a murine monoclonal antibody that recognizes the heavily glycosylated mucin-like domain of the Ebola virus virion-attached glycoprotein (GP) and protects animals against lethal viral challenge. Here we present the crystal structure, at 2.0 A, of 13F6-1-2 in complex with its Ebola virus GP peptide epitope. The GP peptide binds in an extended conformation, anchored primarily by interactions with the heavy chain. Two GP residues, Gln P406 and Arg P409, make extensive side-chain hydrogen bond and electrostatic interactions with the antibody and are likely critical for recognition and affinity. The 13F6-1-2 antibody utilizes a rare V lambda(x) light chain. The three light-chain complementarity-determining regions do not adopt canonical conformations and represent new classes of structures distinct from V kappa and other V lambda light chains. In addition, although V lambda(x) had been thought to confer specificity, all light-chain contacts are mediated through germ-line-encoded residues. This structure of an antibody that protects against the Ebola virus now provides a framework for humanization and development of a postexposure immunotherapeutic.
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===Crystal structure of the 13F6-1-2 Fab fragment bound to its Ebola virus glycoprotein peptide epitope.===
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Complex of a protective antibody with its Ebola virus GP peptide epitope: unusual features of a V lambda x light chain.,Lee JE, Kuehne A, Abelson DM, Fusco ML, Hart MK, Saphire EO J Mol Biol. 2008 Jan 4;375(1):202-16. Epub 2007 Oct 16. PMID:18005986<ref>PMID:18005986</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2qhr" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18005986}}, adds the Publication Abstract to the page
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18005986 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18005986}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QHR OCA].
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[[Category: Mus musculus]]
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[[Category: Abelson DM]]
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==Reference==
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[[Category: Fusco ML]]
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Complex of a protective antibody with its Ebola virus GP peptide epitope: unusual features of a V lambda x light chain., Lee JE, Kuehne A, Abelson DM, Fusco ML, Hart MK, Saphire EO, J Mol Biol. 2008 Jan 4;375(1):202-16. Epub 2007 Oct 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18005986 18005986]
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[[Category: Hart MK]]
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[[Category: Abelson, D M.]]
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[[Category: Kuehne A]]
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[[Category: Fusco, M L.]]
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[[Category: Lee JE]]
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[[Category: Hart, M K.]]
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[[Category: Saphire EO]]
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[[Category: Kuehne, A.]]
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[[Category: Lee, J E.]]
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[[Category: Saphire, E O.]]
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[[Category: Antibody-peptide complex]]
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[[Category: Immune system/viral protein complex]]
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[[Category: Immunologlobulin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 15 10:21:48 2008''
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Current revision

Crystal structure of the 13F6-1-2 Fab fragment bound to its Ebola virus glycoprotein peptide epitope.

PDB ID 2qhr

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