1jc6

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(New page: 200px<br /><applet load="1jc6" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jc6" /> '''SOLUTION STRUCTURE OF BUNGARUS FACIATUS IX, ...)
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[[Image:1jc6.gif|left|200px]]<br /><applet load="1jc6" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jc6" />
 
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'''SOLUTION STRUCTURE OF BUNGARUS FACIATUS IX, A KUNITZ-TYPE CHYMOTRYPSIN INHIBITOR'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF BUNGARUS FACIATUS IX, A KUNITZ-TYPE CHYMOTRYPSIN INHIBITOR==
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Bungarus fasciatus fraction IX (BF9), a chymotrypsin inhibitor, consists, of 65 amino acid residues with three disulfide bridges. It was isolated, from the snake venom of B. fasciatus by ion-exchange chromatography and, belongs to the bovine pancreatic trypsin inhibitor (BPTI)-like, superfamily. It showed a dissociation constant of 5.8 x 10(-8) m with, alpha-chymotrypsin as measured by a BIAcore binding assay system. The, isothermal titration calorimetry revealed a 1:1 binding stoichiometry, between this inhibitor and chymotrypsin and apparently no binding with, trypsin. We further used CD and NMR to determine the solution structure of, this venom-derived chymotrypsin inhibitor. The three-dimensional NMR, solution structures of BF9 were determined on the basis of 582 restraints, by simulated annealing and energy minimization calculations. The final set, of 10 NMR structures was well defined, with average root mean square, deviations of 0.47 A for the backbone atoms in the secondary structure, regions and 0.86 A for residues The side chains of Phe(23), Tyr(24), Tyr(25), Phe(35), and Phe(47) exhibited many long-range nuclear Overhauser, effects and were the principal components of the hydrophobic core in BF9., To gain insight into the structure-function relationships among proteins, in the BPTI-like superfamily, we compared the three-dimensional structure, of BF9 with three BPTI-like proteins that possess distinct biological, functions. These proteins possessed similar secondary structure elements, but the loop regions and beta-turn were different from one another. Based, on residues at the functional site of each protein, we suggest that the, flexibility, rigidity, and variations of the amino acid residues in both, the loop and beta-turn regions are related to their biological functions.
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<StructureSection load='1jc6' size='340' side='right'caption='[[1jc6]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1jc6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bungarus_fasciatus Bungarus fasciatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JC6 FirstGlance]. <br>
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1JC6 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bungarus_fasciatus Bungarus fasciatus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JC6 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jc6 OCA], [https://pdbe.org/1jc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jc6 RCSB], [https://www.ebi.ac.uk/pdbsum/1jc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jc6 ProSAT]</span></td></tr>
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==Reference==
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</table>
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Solution structure of a Kunitz-type chymotrypsin inhibitor isolated from the elapid snake Bungarus fasciatus., Chen C, Hsu CH, Su NY, Lin YC, Chiou SH, Wu SH, J Biol Chem. 2001 Nov 30;276(48):45079-87. Epub 2001 Sep 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11562364 11562364]
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== Function ==
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[https://www.uniprot.org/uniprot/VKT9_BUNFA VKT9_BUNFA] Dual-function toxin that inhibits both serine proteases (high activity on chymotrypsin (Ki = 18 nM), and low activity on elastase) and voltage-gated potassium channels Kv1.3/KCNA3 (IC(50) = 120.0 nM).<ref>PMID:11562364</ref> <ref>PMID:24243656</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jc/1jc6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jc6 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bungarus fasciatus]]
[[Category: Bungarus fasciatus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chen, C.]]
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[[Category: Chen C]]
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[[Category: Chiou, S.H.]]
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[[Category: Chiou SH]]
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[[Category: Hsu, C.H.]]
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[[Category: Hsu CH]]
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[[Category: Su, N.Y.]]
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[[Category: Su NY]]
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[[Category: Wu, S.H.]]
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[[Category: Wu SH]]
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[[Category: bf ix]]
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[[Category: chymotrypsin inhibitor]]
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[[Category: kunitz inhibitor]]
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[[Category: neurotoxin]]
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[[Category: nmr]]
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[[Category: protease inhibitor]]
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[[Category: snake venom]]
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[[Category: solution structure]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:06:05 2007''
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Current revision

SOLUTION STRUCTURE OF BUNGARUS FACIATUS IX, A KUNITZ-TYPE CHYMOTRYPSIN INHIBITOR

PDB ID 1jc6

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