1wa5

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:26, 13 December 2023) (edit) (undo)
 
(9 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:1wa5.png|left|200px]]
 
-
<!--
+
==Structure of the Cse1:Imp-alpha:RanGTP complex==
-
The line below this paragraph, containing "STRUCTURE_1wa5", creates the "Structure Box" on the page.
+
<StructureSection load='1wa5' size='340' side='right'caption='[[1wa5]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1wa5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The January 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Importins'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_1 10.2210/rcsb_pdb/mom_2007_1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WA5 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-
{{STRUCTURE_1wa5| PDB=1wa5 | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wa5 OCA], [https://pdbe.org/1wa5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wa5 RCSB], [https://www.ebi.ac.uk/pdbsum/1wa5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wa5 ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/CSE1_YEAST CSE1_YEAST] Export receptor for importin alpha (SRP1). Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates have been released into the nucleoplasm.<ref>PMID:9744791</ref> <ref>PMID:9857050</ref> <ref>PMID:9774694</ref> <ref>PMID:10394916</ref>
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/1wa5_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wa5 ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The nuclear import and export of macromolecular cargoes through nuclear pore complexes is mediated primarily by carriers such as importin-beta. Importins carry cargoes into the nucleus, whereas exportins carry cargoes to the cytoplasm. Transport is orchestrated by nuclear RanGTP, which dissociates cargoes from importins, but conversely is required for cargo binding to exportins. Here we present the 2.0 A crystal structure of the nuclear export complex formed by exportin Cse1p complexed with its cargo (Kap60p) and RanGTP, thereby providing a structural framework for understanding nuclear protein export and the different functions of RanGTP in export and import. In the complex, Cse1p coils around both RanGTP and Kap60p, stabilizing the RanGTP-state and clamping the Kap60p importin-beta-binding domain, ensuring that only cargo-free Kap60p is exported. Mutagenesis indicated that conformational changes in exportins couple cargo binding to high affinity for RanGTP, generating a spring-loaded molecule to facilitate disassembly of the export complex following GTP hydrolysis in the cytoplasm.
-
===CRYSTAL STRUCTURE OF THE EXPORTIN CSE1P COMPLEXED WITH ITS CARGO (KAP60P) AND RANGTP===
+
Structural basis for the assembly of a nuclear export complex.,Matsuura Y, Stewart M Nature. 2004 Dec 16;432(7019):872-7. PMID:15602554<ref>PMID:15602554</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 1wa5" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_15602554}}, adds the Publication Abstract to the page
+
*[[Importin 3D structures|Importin 3D structures]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 15602554 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_15602554}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
1WA5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Additional information on 1WA5 is available in a page on [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb85_1.html Importins] at the RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WA5 OCA].
+
-
 
+
-
==Reference==
+
-
Structural basis for the assembly of a nuclear export complex., Matsuura Y, Stewart M, Nature. 2004 Dec 16;432(7019):872-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15602554 15602554]
+
[[Category: Canis lupus familiaris]]
[[Category: Canis lupus familiaris]]
[[Category: Importins]]
[[Category: Importins]]
-
[[Category: Protein complex]]
+
[[Category: Large Structures]]
 +
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Matsuura, Y.]]
+
[[Category: Stewart M]]
-
[[Category: Stewart, M.]]
+
-
[[Category: Exportin]]
+
-
[[Category: Importin alpha]]
+
-
[[Category: Nuclear transport]]
+
-
[[Category: Protein transport]]
+
-
[[Category: Ran gtpase]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 10:53:08 2008''
+

Current revision

Structure of the Cse1:Imp-alpha:RanGTP complex

PDB ID 1wa5

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wa5"
Personal tools