1jfg

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(New page: 200px<br /><applet load="1jfg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jfg, resolution 2.5&Aring;" /> '''TRICHODIENE SYNTHASE ...)
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[[Image:1jfg.jpg|left|200px]]<br /><applet load="1jfg" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jfg, resolution 2.5&Aring;" />
 
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'''TRICHODIENE SYNTHASE FROM FUSARIUM SPOROTRICHIOIDES COMPLEXED WITH DIPHOSPHATE'''<br />
 
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==Overview==
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==TRICHODIENE SYNTHASE FROM FUSARIUM SPOROTRICHIOIDES COMPLEXED WITH DIPHOSPHATE==
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The x-ray crystal structure of recombinant trichodiene synthase from, Fusarium sporotrichioides has been determined to 2.5-A resolution, both, unliganded and complexed with inorganic pyrophosphate. This reaction, product coordinates to three Mg(2+) ions near the mouth of the active site, cleft. A comparison of the liganded and unliganded structures reveals a, ligand-induced conformational change that closes the mouth of the active, site cleft. Binding of the substrate farnesyl diphosphate similarly may, trigger this conformational change, which would facilitate catalysis by, protecting reactive carbocationic intermediates in the cyclization, cascade. Trichodiene synthase also shares significant structural, similarity with other sesquiterpene synthases despite a lack of, significant sequence identity. This similarity indicates divergence from a, common ancestor early in the evolution of terpene biosynthesis.
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<StructureSection load='1jfg' size='340' side='right'caption='[[1jfg]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jfg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JFG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JFG FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jfg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jfg OCA], [https://pdbe.org/1jfg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jfg RCSB], [https://www.ebi.ac.uk/pdbsum/1jfg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jfg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRI5_FUSSP TRI5_FUSSP] TS is a member of the terpene cyclase group of enzymes. It catalyzes the isomerization and cyclization of farnesyl pyro-phosphate to form trichodiene, the first cyclic intermediate in the biosynthetic pathway for trichothecenes. It serves to branch trichothecene biosynthesis from the isoprenoid pathway.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jf/1jfg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jfg ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The x-ray crystal structure of recombinant trichodiene synthase from Fusarium sporotrichioides has been determined to 2.5-A resolution, both unliganded and complexed with inorganic pyrophosphate. This reaction product coordinates to three Mg(2+) ions near the mouth of the active site cleft. A comparison of the liganded and unliganded structures reveals a ligand-induced conformational change that closes the mouth of the active site cleft. Binding of the substrate farnesyl diphosphate similarly may trigger this conformational change, which would facilitate catalysis by protecting reactive carbocationic intermediates in the cyclization cascade. Trichodiene synthase also shares significant structural similarity with other sesquiterpene synthases despite a lack of significant sequence identity. This similarity indicates divergence from a common ancestor early in the evolution of terpene biosynthesis.
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==About this Structure==
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Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade.,Rynkiewicz MJ, Cane DE, Christianson DW Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13543-8. Epub 2001 Nov 6. PMID:11698643<ref>PMID:11698643</ref>
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1JFG is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Fusarium_sporotrichioides Fusarium sporotrichioides] with MG, POP and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Trichodiene_synthase Trichodiene synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.3.6 4.2.3.6] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JFG OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade., Rynkiewicz MJ, Cane DE, Christianson DW, Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13543-8. Epub 2001 Nov 6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11698643 11698643]
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</div>
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[[Category: Fusarium sporotrichioides]]
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<div class="pdbe-citations 1jfg" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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[[Category: Trichodiene synthase]]
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[[Category: Cane, D.E.]]
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[[Category: Christianson, D.W.]]
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[[Category: Rynkiewicz, M.J.]]
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[[Category: GOL]]
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[[Category: MG]]
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[[Category: POP]]
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[[Category: terpenoid synthase fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:11:47 2007''
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==See Also==
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*[[Trichodiene synthase|Trichodiene synthase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Fusarium sporotrichioides]]
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[[Category: Large Structures]]
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[[Category: Cane DE]]
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[[Category: Christianson DW]]
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[[Category: Rynkiewicz MJ]]

Current revision

TRICHODIENE SYNTHASE FROM FUSARIUM SPOROTRICHIOIDES COMPLEXED WITH DIPHOSPHATE

PDB ID 1jfg

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