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1gco

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{{Seed}}
 
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[[Image:1gco.png|left|200px]]
 
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==CRYSTAL STRUCTURE OF GLUCOSE DEHYDROGENASE COMPLEXED WITH NAD+==
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The line below this paragraph, containing "STRUCTURE_1gco", creates the "Structure Box" on the page.
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<StructureSection load='1gco' size='340' side='right'caption='[[1gco]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1gco]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. The May 2006 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Glucose Oxidase'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2006_5 10.2210/rcsb_pdb/mom_2006_5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GCO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GCO FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
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{{STRUCTURE_1gco| PDB=1gco | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gco FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gco OCA], [https://pdbe.org/1gco PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gco RCSB], [https://www.ebi.ac.uk/pdbsum/1gco PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gco ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DHG_PRIMG DHG_PRIMG]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gc/1gco_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1gco ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The crystal structure of glucose dehydrogenase (GlcDH) from Bacillus megaterium IWG3 has been determined to an R-factor of 17.9% at 1.7 A resolution. The enzyme consists of four identical subunits, which are similar to those of other short-chain reductases/dehydrogenases (SDRs) in their overall folding and subunit architecture, although cofactor binding sites and subunit interactions differ. Whereas a pair of basic residues is well conserved among NADP(+)-preferring SDRs, only Arg39 was found around the adenine ribose moiety of GlcDH. This suggests that one basic amino acid is enough to determine the coenzyme specificity. The four subunits are interrelated by three mutually perpendicular diad axes (P, Q, and R). While subunit interactions through the P-axis for GlcDH are not so different from those of the other SDRs, those through the Q-axis differ significantly. GlcDH was found to have weaker hydrophobic interactions in the Q-interface. Moreover, GlcDH lacks the salt bridge that stabilizes the subunit interaction in the Q-interface in the other SDRs. Hydrogen bonds between Q-axis related subunits are also less common than in the other SDRs. The GlcDH tetramer dissociates into inactive monomers at pH 9.0, which can be attributed mainly to the weakness of the Q-axis interface.
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===CRYSTAL STRUCTURE OF GLUCOSE DEHYDROGENASE COMPLEXED WITH NAD+===
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Crystal structure of glucose dehydrogenase from Bacillus megaterium IWG3 at 1.7 A resolution.,Yamamoto K, Kurisu G, Kusunoki M, Tabata S, Urabe I, Osaki S J Biochem. 2001 Feb;129(2):303-12. PMID:11173533<ref>PMID:11173533</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_11173533}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1gco" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 11173533 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_11173533}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1GCO is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_megaterium Bacillus megaterium]. Additional information on 1GCO is available in a page on [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb77_1.html Glucose Oxidase] at the RCSB PDB [http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GCO OCA].
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==Reference==
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Crystal structure of glucose dehydrogenase from Bacillus megaterium IWG3 at 1.7 A resolution., Yamamoto K, Kurisu G, Kusunoki M, Tabata S, Urabe I, Osaki S, J Biochem. 2001 Feb;129(2):303-12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11173533 11173533]
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[[Category: Bacillus megaterium]]
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[[Category: Glucose 1-dehydrogenase]]
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[[Category: Glucose Oxidase]]
[[Category: Glucose Oxidase]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Kurisu, G.]]
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[[Category: Priestia megaterium]]
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[[Category: Kusunoki, M.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Osaki, S.]]
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[[Category: Kurisu G]]
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[[Category: Tabata, S.]]
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[[Category: Kusunoki M]]
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[[Category: Urabe, I.]]
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[[Category: Osaki S]]
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[[Category: Yamamoto, K.]]
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[[Category: Tabata S]]
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[[Category: Oxidoreductase]]
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[[Category: Urabe I]]
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[[Category: Short-chain dehydrogenase/reductase]]
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[[Category: Yamamoto K]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 11:12:37 2008''
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CRYSTAL STRUCTURE OF GLUCOSE DEHYDROGENASE COMPLEXED WITH NAD+

PDB ID 1gco

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