2k0a

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{{Seed}}
 
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[[Image:2k0a.jpg|left|200px]]
 
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==1H, 15N and 13C chemical shift assignments for Rds3 protein==
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The line below this paragraph, containing "STRUCTURE_2k0a", creates the "Structure Box" on the page.
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<StructureSection load='2k0a' size='340' side='right'caption='[[2k0a]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2k0a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K0A FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2k0a| PDB=2k0a | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k0a OCA], [https://pdbe.org/2k0a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k0a RCSB], [https://www.ebi.ac.uk/pdbsum/2k0a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k0a ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RDS3_YEAST RDS3_YEAST] Required for pre-mRNA splicing. Involved in regulation of drug sensitivity and may play a role in multidrug resistance.<ref>PMID:11943786</ref> <ref>PMID:14517302</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k0/2k0a_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2k0a ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Rds3p, a component of the U2 snRNP subcomplex SF3b, is essential for pre-mRNA splicing and is extremely well conserved in all eukaryotic species. We report here the solution structure of Rds3p, which reveals an unusual knotted fold unrelated to previously known knotted proteins. Rds3p has a triangular shape with a GATA-like zinc finger at each vertex. Pairs of cysteines contributing to each finger are arranged nonsequentially in a permuted arrangement reminiscent of domain-swapping but which here involves segments of subdomains within a single chain. We suggest that the structure arose through a process of segment swapping after gene duplication. The fingers are connected through beta-strands and loops, forming an overall topology strongly resembling a "triquetra knot." The conservation and surface properties of Rds3p suggest that it functions as a platform for protein assembly within the multiprotein SF3b complex of U2 snRNP. The recombinant protein used for structure determination is biologically active, as it restores splicing activity in a yeast splicing extract depleted of native Rds3p.
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===1H, 15N and 13C chemical shift assignments for Rds3 protein===
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Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif.,van Roon AM, Loening NM, Obayashi E, Yang JC, Newman AJ, Hernandez H, Nagai K, Neuhaus D Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9621-6. Epub 2008 Jul 8. PMID:18621724<ref>PMID:18621724</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2k0a" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_18621724}}, adds the Publication Abstract to the page
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*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 18621724 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_18621724}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2K0A is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K0A OCA].
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==Reference==
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Solution structure of the U2 snRNP protein Rds3p reveals a knotted zinc-finger motif., van Roon AM, Loening NM, Obayashi E, Yang JC, Newman AJ, Hernandez H, Nagai K, Neuhaus D, Proc Natl Acad Sci U S A. 2008 Jul 15;105(28):9621-6. Epub 2008 Jul 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18621724 18621724]
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[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
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[[Category: Single protein]]
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[[Category: Loening N]]
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[[Category: Loening, N.]]
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[[Category: Nagai K]]
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[[Category: Nagai, K.]]
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[[Category: Neuhaus D]]
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[[Category: Neuhaus, D.]]
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[[Category: Yang J]]
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[[Category: Roon, A van.]]
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[[Category: Van Roon A]]
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[[Category: Yang, J.]]
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[[Category: Metal binding protein]]
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[[Category: Mrna processing]]
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[[Category: Mrna splicing]]
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[[Category: Nucleus]]
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[[Category: Rna binding protein]]
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[[Category: Spliceosome]]
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[[Category: Topological knot]]
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[[Category: Zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 11:32:59 2008''
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Current revision

1H, 15N and 13C chemical shift assignments for Rds3 protein

PDB ID 2k0a

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