2z6x

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (13:23, 1 November 2023) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2z6x.jpg|left|200px]]
 
-
<!--
+
==Crystal structure of 22G, the wild-type protein of the photoswitchable GFP-like protein Dronpa==
-
The line below this paragraph, containing "STRUCTURE_2z6x", creates the "Structure Box" on the page.
+
<StructureSection load='2z6x' size='340' side='right'caption='[[2z6x]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2z6x]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pectiniidae Pectiniidae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z6X FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
-
{{STRUCTURE_2z6x| PDB=2z6x | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z6x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z6x OCA], [https://pdbe.org/2z6x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z6x RCSB], [https://www.ebi.ac.uk/pdbsum/2z6x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z6x ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z6/2z6x_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z6x ConSurf].
 +
<div style="clear:both"></div>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
The structural basis for the photochromism in the fluorescent protein Dronpa is poorly understood, because the crystal structures of the bright state of the protein did not provide an answer to the mechanism of the photochromism, and structural determination of the dark state has been elusive. We performed NMR analyses of Dronpa in solution at ambient temperatures to find structural flexibility of the protein in the dark state. Light-induced changes in interactions between the chromophore and beta-barrel are responsible for switching between the two states. In the bright state, the apex of the chromophore tethers to the barrel by a hydrogen bond, and an imidazole ring protruding from the barrel stabilizes the plane of the chromophore. These interactions are disrupted by strong illumination with blue light, and the chromophore, together with a part of the beta-barrel, becomes flexible, leading to a nonradiative decay process.
-
===Crystal structure of 22G, the wild-type protein of the photoswitchable GFP-like protein Dronpa===
+
Light-dependent regulation of structural flexibility in a photochromic fluorescent protein.,Mizuno H, Mal TK, Walchli M, Kikuchi A, Fukano T, Ando R, Jeyakanthan J, Taka J, Shiro Y, Ikura M, Miyawaki A Proc Natl Acad Sci U S A. 2008 Jul 8;105(27):9227-32. Epub 2008 Jun 23. PMID:18574155<ref>PMID:18574155</ref>
 +
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 +
</div>
 +
<div class="pdbe-citations 2z6x" style="background-color:#fffaf0;"></div>
-
<!--
+
==See Also==
-
The line below this paragraph, {{ABSTRACT_PUBMED_18574155}}, adds the Publication Abstract to the page
+
*[[Dronpa|Dronpa]]
-
(as it appears on PubMed at http://www.pubmed.gov), where 18574155 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_18574155}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
[[Category: Large Structures]]
-
2Z6X is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pectiniidae Pectiniidae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z6X OCA].
+
-
 
+
-
==Reference==
+
-
Light-dependent regulation of structural flexibility in a photochromic fluorescent protein., Mizuno H, Mal TK, Walchli M, Kikuchi A, Fukano T, Ando R, Jeyakanthan J, Taka J, Shiro Y, Ikura M, Miyawaki A, Proc Natl Acad Sci U S A. 2008 Jul 8;105(27):9227-32. Epub 2008 Jun 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18574155 18574155]
+
[[Category: Pectiniidae]]
[[Category: Pectiniidae]]
-
[[Category: Single protein]]
+
[[Category: Jeyakanthan J]]
-
[[Category: Jeyakanthan, J.]]
+
[[Category: Kikuchi A]]
-
[[Category: Kikuchi, A.]]
+
[[Category: Miyawaki A]]
-
[[Category: Miyawaki, A.]]
+
[[Category: Mizuno H]]
-
[[Category: Mizuno, H.]]
+
[[Category: Shiro Y]]
-
[[Category: Shiro, Y.]]
+
[[Category: Taka J]]
-
[[Category: Taka, J.]]
+
-
[[Category: Dronpa]]
+
-
[[Category: Fluorescent protein]]
+
-
[[Category: Gfp-like protein]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 23 11:43:40 2008''
+

Current revision

Crystal structure of 22G, the wild-type protein of the photoswitchable GFP-like protein Dronpa

PDB ID 2z6x

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2z6x"
Personal tools