1jj4

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(New page: 200px<br /><applet load="1jj4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jj4, resolution 2.40&Aring;" /> '''Human papillomavirus...)
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[[Image:1jj4.gif|left|200px]]<br /><applet load="1jj4" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jj4, resolution 2.40&Aring;" />
 
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'''Human papillomavirus type 18 E2 DNA-binding domain bound to its DNA target'''<br />
 
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==Overview==
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==Human papillomavirus type 18 E2 DNA-binding domain bound to its DNA target==
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The papillomavirus E2 proteins regulate the transcription of all, papillomavirus genes and are necessary for viral DNA replication., Disruption of the E2 gene is commonly associated with malignancy in, cervical carcinoma, indicating that E2 has a role in regulating tumor, progression. Although the E2 proteins from all characterized, papillomaviruses bind specifically to the same 12-base pair DNA sequence, the cancer-associated human papillomavirus E2 proteins display a unique, ability to detect DNA flexibility and intrinsic curvature. To understand, the structural basis for this phenomenon, we have determined the crystal, structures of the human papillomavirus-18 E2 DNA-binding domain and its, complexes with high and low affinity binding sites. The E2 protein is a, dimeric beta-barrel and the E2-DNA interaction is accompanied by a large, deformation of the DNA as it conforms to the E2 surface. DNA conformation, and E2-DNA contacts are similar in both high and low affinity complexes., The differences in affinity correlate with the flexibility of the DNA, sequence. Preferences of E2 proteins from different papillomavirus strains, for flexible or prevent DNA targets correlate with the distribution of, positive charge on their DNA interaction surfaces, suggesting a role for, electrostatic forces in the recognition of DNA deformability.
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<StructureSection load='1jj4' size='340' side='right'caption='[[1jj4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jj4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_papillomavirus_type_18 Human papillomavirus type 18]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JJ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JJ4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jj4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jj4 OCA], [https://pdbe.org/1jj4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jj4 RCSB], [https://www.ebi.ac.uk/pdbsum/1jj4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jj4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/VE2_HPV18 VE2_HPV18] E2 regulates viral transcription and DNA replication. It binds to the E2RE response element (5'-ACCNNNNNNGGT-3') present in multiple copies in the regulatory region. It can either activate or repress transcription depending on E2RE's position with regards to proximal promoter elements. Repression occurs by sterically hindering the assembly of the transcription initiation complex. The E1-E2 complex binds to the origin of DNA replication.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jj/1jj4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jj4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JJ4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_papillomavirus_type_63 Human papillomavirus type 63]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JJ4 OCA].
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*[[Regulatory protein E2|Regulatory protein E2]]
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__TOC__
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==Reference==
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</StructureSection>
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The structural basis of DNA target discrimination by papillomavirus E2 proteins., Kim SS, Tam JK, Wang AF, Hegde RS, J Biol Chem. 2000 Oct 6;275(40):31245-54. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10906136 10906136]
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[[Category: Human papillomavirus type 18]]
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[[Category: Human papillomavirus type 63]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Hegde RS]]
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[[Category: Hegde, R.S.]]
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[[Category: Kim S-S]]
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[[Category: Kim, S.S.]]
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[[Category: Tam JK]]
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[[Category: Tam, J.K.]]
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[[Category: Wang AF]]
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[[Category: Wang, A.F.]]
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[[Category: dna-binding domain]]
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[[Category: e2]]
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[[Category: e2-dna complex]]
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[[Category: hpv-18]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:19:04 2007''
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Current revision

Human papillomavirus type 18 E2 DNA-binding domain bound to its DNA target

PDB ID 1jj4

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