1jke

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(New page: 200px<br /><applet load="1jke" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jke, resolution 1.55&Aring;" /> '''D-Tyr tRNATyr deacyl...)
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[[Image:1jke.gif|left|200px]]<br /><applet load="1jke" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jke, resolution 1.55&Aring;" />
 
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'''D-Tyr tRNATyr deacylase from Escherichia coli'''<br />
 
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==Overview==
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==D-Tyr tRNATyr deacylase from Escherichia coli==
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Cell growth inhibition by several d-amino acids can be explained by an in, vivo production of d-aminoacyl-tRNA molecules. Escherichia coli and yeast, cells express an enzyme, d-Tyr-tRNA(Tyr) deacylase, capable of recycling, such d-aminoacyl-tRNA molecules into free tRNA and d-amino acid., Accordingly, upon inactivation of the genes of the above deacylases, the, toxicity of d-amino acids increases. Orthologs of the deacylase are found, in many cells. In this study, the crystallographic structure of dimeric E., coli d-Tyr-tRNA(Tyr) deacylase at 1.55 A resolution is reported. The, structure corresponds to a beta-barrel closed on one side by a beta-sheet, lid. This barrel results from the assembly of the two subunits. Analysis, of the structure in relation with sequence homologies in the orthologous, family suggests the location of the active sites at the carboxy end of the, beta-strands. The solved structure markedly differs from those of all, other documented tRNA-dependent hydrolases.
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<StructureSection load='1jke' size='340' side='right'caption='[[1jke]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1jke]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JKE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JKE FirstGlance]. <br>
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1JKE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JKE OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jke OCA], [https://pdbe.org/1jke PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jke RCSB], [https://www.ebi.ac.uk/pdbsum/1jke PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jke ProSAT]</span></td></tr>
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Structure of crystalline D-Tyr-tRNA(Tyr) deacylase. A representative of a new class of tRNA-dependent hydrolases., Ferri-Fioni ML, Schmitt E, Soutourina J, Plateau P, Mechulam Y, Blanquet S, J Biol Chem. 2001 Dec 14;276(50):47285-90. Epub 2001 Sep 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11568181 11568181]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DTD_ECOLI DTD_ECOLI] Hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). Could be a defense mechanism against a harmful effect of D-tyrosine. Can also cleave D-aspartyl-tRNA(Asp) and D-tryptophanyl-tRNA(Trp).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jk/1jke_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jke ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Blanquet, S.]]
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[[Category: Blanquet S]]
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[[Category: Ferri-Fioni, M.L.]]
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[[Category: Ferri-Fioni ML]]
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[[Category: Mechulam, Y.]]
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[[Category: Mechulam Y]]
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[[Category: Plateau, P.]]
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[[Category: Plateau P]]
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[[Category: Schmitt, E.]]
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[[Category: Schmitt E]]
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[[Category: Soutourina, J.]]
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[[Category: Soutourina J]]
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[[Category: ZN]]
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[[Category: beta-alpha-barrel]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:20:36 2007''
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Current revision

D-Tyr tRNATyr deacylase from Escherichia coli

PDB ID 1jke

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