1qxi
From Proteopedia
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(New page: '''Theoretical Model''' The entry 1QXI is a Theoretical Model titled 'RNA Ligase, class I'. Category:Theoretical Model ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed ...) |
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| - | + | {{Theoretical_model}} | |
| - | + | ==RNA LIGASE, CLASS I== | |
| + | <StructureSection load='1qxi' size='340' side='right'caption='[[1qxi]]' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QXI FirstGlance]. <br> | ||
| + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qxi FirstGlance], [https://www.ebi.ac.uk/pdbsum/1qxi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qxi ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The class I ligase ribozyme catalyzes a Mg(++)-dependent RNA-ligation reaction that is chemically analogous to a single step of RNA polymerization. Indeed, this ribozyme constitutes the catalytic domain of an accurate and general RNA polymerase ribozyme. The ligation reaction is also very rapid in both single- and multiple-turnover contexts and thus is informative for the study of RNA catalysis as well as RNA self-replication. Here we report the initial characterization of the three-dimensional architecture of the ligase. When the ligase folds, several segments become protected from hydroxyl-radical cleavage, indicating that the RNA adopts a compact tertiary structure. Ribozyme folding was largely, though not completely, Mg(++) dependent, with a K(1/2[Mg]) < 1 mM, and was observed over a broad temperature range (20 degrees C -50 degrees C). The hydroxyl-radical mapping, together with comparative sequence analyses and analogy to a region within 23S ribosomal RNA, were used to generate a three-dimensional model of the ribozyme. The predictive value of the model was tested and supported by a photo-cross-linking experiment. | ||
| - | + | The three-dimensional architecture of the class I ligase ribozyme.,Bergman NH, Lau NC, Lehnert V, Westhof E, Bartel DP RNA. 2004 Feb;10(2):176-84. PMID:14730016<ref>PMID:14730016</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| + | <div class="pdbe-citations 1qxi" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Theoretical Model]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Bartel, D P]] | ||
| + | [[Category: Bergman, N H]] | ||
| + | [[Category: Lau, N C]] | ||
| + | [[Category: Lehnert, V]] | ||
| + | [[Category: Westhof, E]] | ||
Current revision
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RNA LIGASE, CLASS I
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