1jl5

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(New page: 200px<br /><applet load="1jl5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jl5, resolution 2.10&Aring;" /> '''Novel Molecular Arch...)
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[[Image:1jl5.jpg|left|200px]]<br /><applet load="1jl5" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jl5, resolution 2.10&Aring;" />
 
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'''Novel Molecular Architecture of YopM-a Leucine-rich Effector Protein from Yersinia pestis'''<br />
 
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==Overview==
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==Novel Molecular Architecture of YopM-a Leucine-rich Effector Protein from Yersinia pestis==
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Many Gram-negative bacterial pathogens employ a contact-dependent (type, III) secretion system to deliver effector proteins into the cytosol of, animal or plant cells. Collectively, these effectors enable the bacteria, to evade the immune response of the infected organism by modulating, host-cell functions. YopM, a member of the leucine-rich repeat protein, superfamily, is an effector produced by the bubonic plague bacterium, Yersinia pestis, that is essential for virulence. Here, we report crystal, structures of YopM at 2.4 and 2.1 A resolution. Among all leucine-rich, repeat family members whose atomic coordinates have been reported, the, repeating unit of YopM has the least canonical secondary structure. In, both crystals, four YopM monomers form a hollow cylinder with an inner, diameter of 35 A. The domain that targets YopM for translocation into, eukaryotic cells adopts a well-ordered, alpha-helical conformation that, packs tightly against the proximal leucine-rich repeat module. A similar, alpha-helical domain can be identified in virulence-associated, leucine-rich repeat proteins produced by Salmonella typhimurium and, Shigella flexneri, and in the conceptual translation products of several, open reading frames in Y. pestis.
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<StructureSection load='1jl5' size='340' side='right'caption='[[1jl5]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jl5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JL5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JL5 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jl5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jl5 OCA], [https://pdbe.org/1jl5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jl5 RCSB], [https://www.ebi.ac.uk/pdbsum/1jl5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jl5 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/YOPM_YERPE YOPM_YERPE] Effector proteins function to alter host cell physiology and promote bacterial survival in host tissues (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jl/1jl5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jl5 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Many Gram-negative bacterial pathogens employ a contact-dependent (type III) secretion system to deliver effector proteins into the cytosol of animal or plant cells. Collectively, these effectors enable the bacteria to evade the immune response of the infected organism by modulating host-cell functions. YopM, a member of the leucine-rich repeat protein superfamily, is an effector produced by the bubonic plague bacterium, Yersinia pestis, that is essential for virulence. Here, we report crystal structures of YopM at 2.4 and 2.1 A resolution. Among all leucine-rich repeat family members whose atomic coordinates have been reported, the repeating unit of YopM has the least canonical secondary structure. In both crystals, four YopM monomers form a hollow cylinder with an inner diameter of 35 A. The domain that targets YopM for translocation into eukaryotic cells adopts a well-ordered, alpha-helical conformation that packs tightly against the proximal leucine-rich repeat module. A similar alpha-helical domain can be identified in virulence-associated leucine-rich repeat proteins produced by Salmonella typhimurium and Shigella flexneri, and in the conceptual translation products of several open reading frames in Y. pestis.
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==About this Structure==
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Unusual molecular architecture of the Yersinia pestis cytotoxin YopM: a leucine-rich repeat protein with the shortest repeating unit.,Evdokimov AG, Anderson DE, Routzahn KM, Waugh DS J Mol Biol. 2001 Sep 28;312(4):807-21. PMID:11575934<ref>PMID:11575934</ref>
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1JL5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Yersinia_pestis Yersinia pestis] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JL5 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Unusual molecular architecture of the Yersinia pestis cytotoxin YopM: a leucine-rich repeat protein with the shortest repeating unit., Evdokimov AG, Anderson DE, Routzahn KM, Waugh DS, J Mol Biol. 2001 Sep 28;312(4):807-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11575934 11575934]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 1jl5" style="background-color:#fffaf0;"></div>
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[[Category: Yersinia pestis]]
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[[Category: Anderson, D.E.]]
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[[Category: Evdokimov, A.G.]]
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[[Category: Routzahn, K.M.]]
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[[Category: Waugh, D.S.]]
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[[Category: CA]]
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[[Category: effector protein]]
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[[Category: leucine-rich repeat]]
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[[Category: molecular pathogenesis]]
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[[Category: virulence factor]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:21:37 2007''
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==See Also==
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*[[Amylase 3D structures|Amylase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Yersinia pestis]]
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[[Category: Anderson DE]]
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[[Category: Evdokimov AG]]
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[[Category: Routzahn KM]]
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[[Category: Waugh DS]]

Current revision

Novel Molecular Architecture of YopM-a Leucine-rich Effector Protein from Yersinia pestis

PDB ID 1jl5

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