1jn2

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(New page: 200px<br /><applet load="1jn2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jn2, resolution 1.9&Aring;" /> '''Crystal Structure of ...)
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[[Image:1jn2.jpg|left|200px]]<br /><applet load="1jn2" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jn2, resolution 1.9&Aring;" />
 
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'''Crystal Structure of meso-tetrasulphonatophenyl porphyrin complexed with Concanavalin A'''<br />
 
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==Overview==
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==Crystal Structure of meso-tetrasulphonatophenyl porphyrin complexed with Concanavalin A==
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The crystal structure of meso-tetrasulfonatophenylporphyrin complexed with, concanavalin A (ConA) was determined at 1.9 A resolution. Comparison of, this structure with that of ConA bound to methyl alpha-d-mannopyranoside, provided direct structural evidence of molecular mimicry in the context of, ligand receptor binding. The sulfonatophenyl group of, meso-tetrasulfonatophenylporphyrin occupies the same binding site on ConA, as that of methyl alpha-d-mannopyranoside, a natural ligand. A pair of, stacked porphyrin molecules stabilizes the crystal structure by end-to-end, cross-linking with ConA resulting in a network similar to that observed, upon agglutination of cells by lectins. The porphyrin binds to ConA, predominantly through hydrogen bonds and water-mediated interactions. The, sandwiched water molecules in the complex play a cementing role, facilitating favorable binding of porphyrin. Seven of the eight hydrogen, bonds observed between methyl alpha-d-mannopyranoside and ConA are, mimicked by the sulfonatophenyl group of porphyrin after incorporating two, water molecules. Thus, the similarity in chemical interactions was, manifested in terms of functional mimicry despite the obvious structural, dissimilarity between the sugar and the porphyrin.
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<StructureSection load='1jn2' size='340' side='right'caption='[[1jn2]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jn2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JN2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JN2 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SFP:5,10,15,20-TETRAKIS(4-SULPFONATOPHENYL)-21H,23H-PORPHINE'>SFP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jn2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jn2 OCA], [https://pdbe.org/1jn2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jn2 RCSB], [https://www.ebi.ac.uk/pdbsum/1jn2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jn2 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CONA_CANEN CONA_CANEN] D-mannose specific lectin.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jn/1jn2_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jn2 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JN2 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Canavalia_ensiformis Canavalia ensiformis] with CA, MN and SFP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JN2 OCA].
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*[[Concanavalin 3D structures|Concanavalin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Functional equality in the absence of structural similarity: an added dimension to molecular mimicry., Goel M, Jain D, Kaur KJ, Kenoth R, Maiya BG, Swamy MJ, Salunke DM, J Biol Chem. 2001 Oct 19;276(42):39277-81. Epub 2001 Aug 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11504727 11504727]
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[[Category: Canavalia ensiformis]]
[[Category: Canavalia ensiformis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Goel, M.]]
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[[Category: Goel M]]
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[[Category: Jain, D.]]
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[[Category: Jain D]]
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[[Category: Kaur, K.J.]]
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[[Category: Kaur KJ]]
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[[Category: Kenoth, R.]]
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[[Category: Kenoth R]]
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[[Category: Maiya, B.G.]]
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[[Category: Maiya BG]]
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[[Category: Salunke, D.M.]]
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[[Category: Salunke DM]]
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[[Category: Swamy, M.J.]]
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[[Category: Swamy MJ]]
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[[Category: CA]]
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[[Category: MN]]
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[[Category: SFP]]
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[[Category: lectin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:23:43 2007''
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Current revision

Crystal Structure of meso-tetrasulphonatophenyl porphyrin complexed with Concanavalin A

PDB ID 1jn2

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