1jth

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(New page: 200px<br /><applet load="1jth" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jth, resolution 2.00&Aring;" /> '''Crystal structure an...)
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[[Image:1jth.gif|left|200px]]<br /><applet load="1jth" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jth, resolution 2.00&Aring;" />
 
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'''Crystal structure and biophysical properties of a complex between the N-terminal region of SNAP25 and the SNARE region of syntaxin 1a'''<br />
 
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==Overview==
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==Crystal structure and biophysical properties of a complex between the N-terminal region of SNAP25 and the SNARE region of syntaxin 1a==
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SNARE proteins are required for intracellular membrane fusion. In the, neuron, the plasma membrane SNAREs syntaxin 1a and SNAP25 bind to VAMP2, found on neurotransmitter-containing vesicles. These three proteins, contain "SNARE regions" that mediate their association into stable, tetrameric coiled-coil structures. Syntaxin 1a contributes one such, region, designated H3, and SNAP25 contributes two SNARE regions to the, fusogenic complex with VAMP2. Syntaxin 1a H3 (syn1aH3) and SNAP25 can form, a stable assembly, which can then be bound by VAMP2 to form the full SNARE, complex. Here we show that syn1aH3 can also form a stable but kinetically, trapped complex with the N-terminal SNARE region of SNAP25 (S25N). The, crystal structure of this complex reveals an extended parallel four-helix, bundle similar to that of the core SNARE and the syn1aH3-SNAP25 complexes., The inherent ability of syn1aH3 and S25N to associate stably in vitro, implies that the intracellular fusion machinery must prevent formation of, or remove, any non-productive complexes. Comparison with the, syn1aH3-SNAP25 complex suggests that the linkage of the N- and C-terminal, SNAP25 SNARE regions is kinetically advantageous in preventing formation, of the non-productive syn1aH3-S25N complex. We also demonstrate that the, syn1aH3-S25N complex can be disassembled by alpha-SNAP and, N-ethylmaleimide-sensitive factor.
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<StructureSection load='1jth' size='340' side='right'caption='[[1jth]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jth]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JTH FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jth OCA], [https://pdbe.org/1jth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jth RCSB], [https://www.ebi.ac.uk/pdbsum/1jth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jth ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/SNP25_RAT SNP25_RAT] t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jth_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jth ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JTH is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JTH OCA].
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*[[Synaptosomal-associated protein|Synaptosomal-associated protein]]
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*[[Syntaxin 3D structures|Syntaxin 3D structures]]
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==Reference==
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__TOC__
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Crystal structure and biophysical properties of a complex between the N-terminal SNARE region of SNAP25 and syntaxin 1a., Misura KM, Gonzalez LC Jr, May AP, Scheller RH, Weis WI, J Biol Chem. 2001 Nov 2;276(44):41301-9. Epub 2001 Aug 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11533035 11533035]
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</StructureSection>
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[[Category: Protein complex]]
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Jr., L.C.Gonzalez.]]
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[[Category: Gonzalez Jr LC]]
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[[Category: May, A.P.]]
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[[Category: May AP]]
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[[Category: Misura, K.M.S.]]
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[[Category: Misura KMS]]
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[[Category: Scheller, R.H.]]
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[[Category: Scheller RH]]
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[[Category: Weis, W.I.]]
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[[Category: Weis WI]]
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[[Category: coiled-coil]]
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[[Category: polar layer]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:34:00 2007''
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Current revision

Crystal structure and biophysical properties of a complex between the N-terminal region of SNAP25 and the SNARE region of syntaxin 1a

PDB ID 1jth

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