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1jti

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(New page: 200px<br /><applet load="1jti" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jti, resolution 2.30&Aring;" /> '''Loop-inserted Struct...)
Current revision (07:54, 3 April 2024) (edit) (undo)
 
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[[Image:1jti.gif|left|200px]]<br /><applet load="1jti" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jti, resolution 2.30&Aring;" />
 
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'''Loop-inserted Structure of P1-P1' Cleaved Ovalbumin Mutant R339T'''<br />
 
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==Overview==
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==Loop-inserted Structure of P1-P1' Cleaved Ovalbumin Mutant R339T==
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Ovalbumin is a member of a superfamily of serine proteinase inhibitors, known as the serpins. It is, however, non-inhibitory towards serine, proteinases, and lacks the loop insertion mechanism common to the serpins, due to unknown structural factors. Mutant ovalbumin, R339T, in which the, P14 hinge residue is replaced, was produced and analyzed for its, thermostability and three-dimensional structure. Differential scanning, calorimetry revealed that the mutant ovalbumin, but not the wild-type, protein, undergoes a marked thermostabilization (DeltaT(m)=15.8 degrees C), following the P1-P1' cleavage. Furthermore, the crystal structure, solved, at 2.3 A resolution, clearly proved that the P1-P1' cleaved form assumes, the fully loop-inserted conformation as seen in serpin that possess, inhibitory activity. We therefore conclude that ovalbumin acquires the, structural transition mechanism into the loop-inserted, thermostabilized, form by the single hinge mutation. The mutant protein does not, however, possess inhibitory activity. The solved structure displays the occurrence, of specific interactions that may prevent the smooth motion, relative to, sheet A, of helices E and F and of the loop that follows helix F. These, observations provide crucial insights into the question why R339T is still, non-inhibitory.
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<StructureSection load='1jti' size='340' side='right'caption='[[1jti]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jti]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JTI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JTI FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jti FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jti OCA], [https://pdbe.org/1jti PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jti RCSB], [https://www.ebi.ac.uk/pdbsum/1jti PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jti ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/OVAL_CHICK OVAL_CHICK] Non-inhibitory serpin. Storage protein of egg white.<ref>PMID:6749856</ref> <ref>PMID:3732511</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jt/1jti_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jti ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JTI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JTI OCA].
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*[[Ovalbumin|Ovalbumin]]
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== References ==
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==Reference==
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<references/>
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Loop-inserted and thermostabilized structure of P1-P1' cleaved ovalbumin mutant R339T., Yamasaki M, Arii Y, Mikami B, Hirose M, J Mol Biol. 2002 Jan 11;315(2):113-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11779232 11779232]
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__TOC__
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</StructureSection>
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Arii, Y.]]
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[[Category: Arii Y]]
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[[Category: Hirose, M.]]
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[[Category: Hirose M]]
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[[Category: Mikami, B.]]
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[[Category: Mikami B]]
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[[Category: Yamasaki, M.]]
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[[Category: Yamasaki M]]
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[[Category: loop insertion]]
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[[Category: non-inhibitory serpin]]
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[[Category: ovalbumin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:34:04 2007''
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Current revision

Loop-inserted Structure of P1-P1' Cleaved Ovalbumin Mutant R339T

PDB ID 1jti

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