1nzj

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{{Seed}}
 
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[[Image:1nzj.png|left|200px]]
 
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==Crystal Structure and Activity Studies of Escherichia Coli Yadb ORF==
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The line below this paragraph, containing "STRUCTURE_1nzj", creates the "Structure Box" on the page.
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<StructureSection load='1nzj' size='340' side='right'caption='[[1nzj]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1nzj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NZJ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1nzj| PDB=1nzj | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nzj OCA], [https://pdbe.org/1nzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nzj RCSB], [https://www.ebi.ac.uk/pdbsum/1nzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nzj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLUQ_ECOLI GLUQ_ECOLI] Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5-dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in position 34 of the tRNA(Asp), the wobble position of the QUC anticodon. Does not transfer glutamate to either tRNA(Glu) or tRNA(Gln). The incapacity of the glutamylated tRNA(Asp) to bind elongation factor Tu suggests that it is not involved in ribosomal protein biosynthesis.<ref>PMID:15096594</ref> <ref>PMID:15096612</ref> <ref>PMID:15150343</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/nz/1nzj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nzj ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In the course of a structural genomics program aiming at solving the structures of Escherichia coli open reading frame products of unknown function, we have determined the structure of YadB at 1.5A using molecular replacement. The YadB protein is 298 amino acid residues long and displays 34% sequence identity with E.coli glutamyl-tRNA synthetase (GluRS). It is much shorter than GluRS, which contains 468 residues, and lacks the complete domain interacting with the tRNA anticodon loop. As E.coli GluRS, YadB possesses a Zn2+ located in the putative tRNA acceptor stem-binding domain. The YadB cluster uses cysteine residues as the first three zinc ligands, but has a weaker tyrosine ligand at the fourth position. It shares with canonical amino acid RNA synthetases a major functional feature, namely activation of the amino acid (here glutamate). It differs, however, from GluRSs by the fact that the activation step is tRNA-independent and that it does not catalyze attachment of the activated glutamate to E.coli tRNAGlu, but to another, as yet unknown tRNA. These results suggest thus a novel function, distinct from that of GluRSs, for the yadB gene family.
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===Crystal Structure and Activity Studies of Escherichia Coli Yadb ORF===
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The Escherichia coli YadB gene product reveals a novel aminoacyl-tRNA synthetase like activity.,Campanacci V, Dubois DY, Becker HD, Kern D, Spinelli S, Valencia C, Pagot F, Salomoni A, Grisel S, Vincentelli R, Bignon C, Lapointe J, Giege R, Cambillau C J Mol Biol. 2004 Mar 19;337(2):273-83. PMID:15003446<ref>PMID:15003446</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_15003446}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 1nzj" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 15003446 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_15003446}}
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__TOC__
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</StructureSection>
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==About this Structure==
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1NZJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZJ OCA].
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==Reference==
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The Escherichia coli YadB gene product reveals a novel aminoacyl-tRNA synthetase like activity., Campanacci V, Dubois DY, Becker HD, Kern D, Spinelli S, Valencia C, Pagot F, Salomoni A, Grisel S, Vincentelli R, Bignon C, Lapointe J, Giege R, Cambillau C, J Mol Biol. 2004 Mar 19;337(2):273-83. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15003446 15003446]
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[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Becker, H D.]]
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[[Category: Becker HD]]
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[[Category: Bignon, C.]]
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[[Category: Bignon C]]
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[[Category: Cambillau, C.]]
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[[Category: Cambillau C]]
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[[Category: Campanacci, V.]]
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[[Category: Campanacci V]]
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[[Category: Giege, R.]]
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[[Category: Giege R]]
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[[Category: Kern, D Y.]]
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[[Category: Kern DY]]
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[[Category: Pagot, F.]]
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[[Category: Pagot F]]
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[[Category: Spinelli, S.]]
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[[Category: Spinelli S]]
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[[Category: Valencia, C.]]
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[[Category: Valencia C]]
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[[Category: Vincentelli, R.]]
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[[Category: Vincentelli R]]
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[[Category: Glutamyl t-rna synthetase]]
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[[Category: Structural genomic]]
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[[Category: Zn cluster]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:40:28 2008''
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Current revision

Crystal Structure and Activity Studies of Escherichia Coli Yadb ORF

PDB ID 1nzj

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