1jvv

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(New page: 200px<br /><applet load="1jvv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jvv, resolution 2.20&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1jvv.jpg|left|200px]]<br /><applet load="1jvv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jvv, resolution 2.20&Aring;" />
 
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'''CRYSTAL STRUCTURE OF RIBONUCLEASE A (RETRO-SOAKED FORM)'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF RIBONUCLEASE A (RETRO-SOAKED FORM)==
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Despite the increasing number of successful determinations of complex, protein structures the understanding of their dynamics properties is still, rather limited. Using X-ray crystallography, we demonstrate that, ribonuclease A (RNase A) undergoes significant domain motions upon ligand, binding. In particular, when cytidine 2'-monophosphate binds to RNase A, the structure of the enzyme becomes more compact. Interestingly, our data, also show that these structural alterations are fully reversible in the, crystal state. These findings provide structural bases for the dynamic, behavior of RNase A in the binding of the substrate shown by Petsko and, coworkers (Rasmussen et al. Nature 1992;357:423-424). These subtle domain, motions may assume functional relevance for more complex system and may, play a significant role in the cooperativity of oligomeric enzymes.
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<StructureSection load='1jvv' size='340' side='right'caption='[[1jvv]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jvv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JVV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JVV FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jvv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jvv OCA], [https://pdbe.org/1jvv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jvv RCSB], [https://www.ebi.ac.uk/pdbsum/1jvv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jvv ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jv/1jvv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jvv ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Despite the increasing number of successful determinations of complex protein structures the understanding of their dynamics properties is still rather limited. Using X-ray crystallography, we demonstrate that ribonuclease A (RNase A) undergoes significant domain motions upon ligand binding. In particular, when cytidine 2'-monophosphate binds to RNase A, the structure of the enzyme becomes more compact. Interestingly, our data also show that these structural alterations are fully reversible in the crystal state. These findings provide structural bases for the dynamic behavior of RNase A in the binding of the substrate shown by Petsko and coworkers (Rasmussen et al. Nature 1992;357:423-424). These subtle domain motions may assume functional relevance for more complex system and may play a significant role in the cooperativity of oligomeric enzymes.
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==About this Structure==
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Reversible substrate-induced domain motions in ribonuclease A.,Vitagliano L, Merlino A, Zagari A, Mazzarella L Proteins. 2002 Jan 1;46(1):97-104. PMID:11746706<ref>PMID:11746706</ref>
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1JVV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JVV OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Reversible substrate-induced domain motions in ribonuclease A., Vitagliano L, Merlino A, Zagari A, Mazzarella L, Proteins. 2002 Jan 1;46(1):97-104. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11746706 11746706]
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</div>
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[[Category: Bos taurus]]
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<div class="pdbe-citations 1jvv" style="background-color:#fffaf0;"></div>
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[[Category: Pancreatic ribonuclease]]
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[[Category: Single protein]]
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[[Category: Mazzarella, L.]]
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[[Category: Merlino, A.]]
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[[Category: Vitagliano, L.]]
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[[Category: Zagari, A.]]
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[[Category: protein dynamics]]
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[[Category: protein structure-function]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:37:11 2007''
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==See Also==
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Bos taurus]]
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[[Category: Large Structures]]
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[[Category: Mazzarella L]]
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[[Category: Merlino A]]
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[[Category: Vitagliano L]]
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[[Category: Zagari A]]

Current revision

CRYSTAL STRUCTURE OF RIBONUCLEASE A (RETRO-SOAKED FORM)

PDB ID 1jvv

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