1jw9

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(New page: 200px<br /><applet load="1jw9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jw9, resolution 1.7&Aring;" /> '''Structure of the Nati...)
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[[Image:1jw9.gif|left|200px]]<br /><applet load="1jw9" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jw9, resolution 1.7&Aring;" />
 
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'''Structure of the Native MoeB-MoaD Protein Complex'''<br />
 
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==Overview==
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==Structure of the Native MoeB-MoaD Protein Complex==
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The activation of ubiquitin and related protein modifiers is catalysed by, members of the E1 enzyme family that use ATP for the covalent, self-attachment of the modifiers to a conserved cysteine. The Escherichia, coli proteins MoeB and MoaD are involved in molybdenum cofactor (Moco), biosynthesis, an evolutionarily conserved pathway. The MoeB- and, E1-catalysed reactions are mechanistically similar, and despite a lack of, sequence similarity, MoaD and ubiquitin display the same fold including a, conserved carboxy-terminal Gly-Gly motif. Similar to the E1 enzymes, MoeB, activates the C terminus of MoaD to form an acyl-adenylate. Subsequently, a sulphurtransferase converts the MoaD acyl-adenylate to a thiocarboxylate, that acts as the sulphur donor during Moco biosynthesis. These findings, suggest that ubiquitin and E1 are derived from two ancestral genes closely, related to moaD and moeB. Here we present the crystal structures of the, MoeB-MoaD complex in its apo, ATP-bound, and MoaD-adenylate forms, and, highlight the functional similarities between the MoeB- and E1-substrate, complexes. These structures provide a molecular framework for, understanding the activation of ubiquitin, Rub, SUMO and the sulphur, incorporation step during Moco and thiamine biosynthesis.
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<StructureSection load='1jw9' size='340' side='right'caption='[[1jw9]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1jw9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JW9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JW9 FirstGlance]. <br>
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1JW9 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JW9 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jw9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jw9 OCA], [https://pdbe.org/1jw9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jw9 RCSB], [https://www.ebi.ac.uk/pdbsum/1jw9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jw9 ProSAT]</span></td></tr>
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Mechanism of ubiquitin activation revealed by the structure of a bacterial MoeB-MoaD complex., Lake MW, Wuebbens MM, Rajagopalan KV, Schindelin H, Nature. 2001 Nov 15;414(6861):325-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11713534 11713534]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MOEB_ECOLI MOEB_ECOLI] Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein MoaD.<ref>PMID:11290749</ref> <ref>PMID:11463785</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jw9_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jw9 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Lake, M.W.]]
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[[Category: Lake MW]]
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[[Category: Rajagopalan, K.V.]]
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[[Category: Rajagopalan KV]]
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[[Category: Schindelin, H.]]
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[[Category: Schindelin H]]
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[[Category: Wuebbens, M.M.]]
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[[Category: Wuebbens MM]]
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[[Category: SO4]]
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[[Category: ZN]]
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[[Category: moeb: modified rossmann fold; (2) cys-x-x-cys zinc-binding motifs; moad: ubiquitin-like fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:37:57 2007''
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Current revision

Structure of the Native MoeB-MoaD Protein Complex

PDB ID 1jw9

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