2uuz

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{{Seed}}
 
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[[Image:2uuz.png|left|200px]]
 
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==Orthorhombic crystal form of GamS from bacteriophage lambda.==
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The line below this paragraph, containing "STRUCTURE_2uuz", creates the "Structure Box" on the page.
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<StructureSection load='2uuz' size='340' side='right'caption='[[2uuz]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2uuz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_Lambda Escherichia virus Lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UUZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uuz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uuz OCA], [https://pdbe.org/2uuz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uuz RCSB], [https://www.ebi.ac.uk/pdbsum/2uuz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uuz ProSAT]</span></td></tr>
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{{STRUCTURE_2uuz| PDB=2uuz | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GAM_LAMBD GAM_LAMBD] Binds to host RecBCD nuclease and inhibits it thereby protecting the viral DNA against recBCD mediated degradation.<ref>PMID:17544443</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uu/2uuz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uuz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In Escherichia coli, RecBCD processes double-stranded DNA breaks during the initial stages of homologous recombination. RecBCD contains helicase and nuclease activities, and unwinds and digests the blunt-ended DNA until a specific eight-nucleotide sequence, Chi, is encountered. Chi modulates the nuclease activity of RecBCD and results in a resected DNA end, which is a substrate for RecA during subsequent steps in recombination. RecBCD also acts as a defence mechanism against bacteriophage infection by digesting linear viral DNA present during virus replication or resulting from the action of restriction endonucleases. To avoid this fate, bacteriophage lambda encodes the gene Gam whose product is an inhibitor of RecBCD. Gam has been shown to bind to RecBCD and inhibit its helicase and nuclease activities. We show that Gam inhibits RecBCD by preventing it from binding DNA. We have solved the crystal structure of Gam from two different crystal forms. Using the published crystal structure of RecBCD in complex with DNA we suggest models for the molecular mechanism of Gam-mediated inhibition of RecBCD. We also propose that Gam could be a mimetic of single-stranded, and perhaps also double-stranded, DNA.
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===ORTHORHOMBIC CRYSTAL FORM OF GAMS FROM BACTERIOPHAGE LAMBDA.===
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The crystal structure of lambda-Gam protein suggests a model for RecBCD inhibition.,Court R, Cook N, Saikrishnan K, Wigley D J Mol Biol. 2007 Aug 3;371(1):25-33. Epub 2007 May 18. PMID:17544443<ref>PMID:17544443</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17544443}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2uuz" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17544443 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17544443}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Escherichia virus Lambda]]
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2UUZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_lambda Enterobacteria phage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UUZ OCA].
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[[Category: Large Structures]]
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[[Category: Cook N]]
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==Reference==
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[[Category: Court RI]]
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The crystal structure of lambda-Gam protein suggests a model for RecBCD inhibition., Court R, Cook N, Saikrishnan K, Wigley D, J Mol Biol. 2007 Aug 3;371(1):25-33. Epub 2007 May 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17544443 17544443]
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[[Category: Saikrishnan K]]
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[[Category: Enterobacteria phage lambda]]
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[[Category: Wigley DB]]
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[[Category: Single protein]]
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[[Category: Cook, N.]]
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[[Category: Court, R I.]]
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[[Category: Saikrishnan, K.]]
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[[Category: Wigley, D B.]]
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[[Category: Bacteriophage lambda]]
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[[Category: Inhibitor]]
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[[Category: Nuclease inhibitor]]
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[[Category: Putative dna mimic]]
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[[Category: Recbcd inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:52:50 2008''
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Current revision

Orthorhombic crystal form of GamS from bacteriophage lambda.

PDB ID 2uuz

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OCA

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