2dwy

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{{Seed}}
 
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[[Image:2dwy.png|left|200px]]
 
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==Crystal Structure Analysis of GGA1-GAE==
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The line below this paragraph, containing "STRUCTURE_2dwy", creates the "Structure Box" on the page.
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<StructureSection load='2dwy' size='340' side='right'caption='[[2dwy]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2dwy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DWY FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dwy OCA], [https://pdbe.org/2dwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dwy RCSB], [https://www.ebi.ac.uk/pdbsum/2dwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dwy ProSAT]</span></td></tr>
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{{STRUCTURE_2dwy| PDB=2dwy | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GGA1_HUMAN GGA1_HUMAN] Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif.<ref>PMID:11301005</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dw/2dwy_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2dwy ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Golgi-localizing, gamma-adaptin ear domain homology, ADP ribosylation factor-binding (GGA) proteins and the adaptor protein (AP) complex, AP-1, are involved in membrane traffic between the trans Golgi network and the endosomes. The gamma-adaptin ear (GAE) domain of GGAs and the gamma1 ear domain of AP-1 interact with an acidic phenylalanine motif found in accessory proteins. The GAE domain of GGA1 (GGA1-GAE) interacts with a WNSF-containing peptide derived from its own hinge region, although the peptide sequence deviates from the standard acidic phenylalanine motif. We report here the structure of GGA1-GAE in complex with the GGA1 hinge peptide, which revealed that the two aromatic side chains of the WNSF sequence fit into a hydrophobic groove formed by aliphatic portions of the side chains of conserved arginine and lysine residues of GGA1-GAE, in a similar manner to the interaction between GGA-GAEs and acidic phenylalanine sequences from the accessory proteins. Fluorescence quenching experiments indicate that the GGA1 hinge region binds to GGA1-GAE and competes with accessory proteins for binding. Taken together with the previous observation that gamma1 ear binds to the GGA1 hinge region, the interaction between the hinge region and the GAE domain underlies the autoregulation of GGA function in clathrin-mediated trafficking through competing with the accessory proteins and the AP-1 complex.
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===Crystal Structure Analysis of GGA1-GAE===
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Molecular basis for autoregulatory interaction between GAE domain and hinge region of GGA1.,Inoue M, Shiba T, Ihara K, Yamada Y, Hirano S, Kamikubo H, Kataoka M, Kawasaki M, Kato R, Nakayama K, Wakatsuki S Traffic. 2007 Jul;8(7):904-13. Epub 2007 May 16. PMID:17506864<ref>PMID:17506864</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17506864}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2dwy" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17506864 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17506864}}
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__TOC__
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</StructureSection>
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==About this Structure==
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2DWY is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DWY OCA].
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==Reference==
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Molecular basis for autoregulatory interaction between GAE domain and hinge region of GGA1., Inoue M, Shiba T, Ihara K, Yamada Y, Hirano S, Kamikubo H, Kataoka M, Kawasaki M, Kato R, Nakayama K, Wakatsuki S, Traffic. 2007 Jul;8(7):904-13. Epub 2007 May 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17506864 17506864]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Ihara, K.]]
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[[Category: Ihara K]]
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[[Category: Inoue, M.]]
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[[Category: Inoue M]]
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[[Category: Kato, R.]]
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[[Category: Kato R]]
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[[Category: Kawasaki, M.]]
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[[Category: Kawasaki M]]
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[[Category: Nakayama, K.]]
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[[Category: Nakayama K]]
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[[Category: Shiba, T.]]
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[[Category: Shiba T]]
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[[Category: Wakatsuki, S.]]
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[[Category: Wakatsuki S]]
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[[Category: Yamada, Y.]]
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[[Category: Yamada Y]]
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[[Category: Adaptin]]
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[[Category: Ig fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 13:53:42 2008''
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Current revision

Crystal Structure Analysis of GGA1-GAE

PDB ID 2dwy

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