1jwx

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(New page: 200px<br /><applet load="1jwx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jwx, resolution 1.63&Aring;" /> '''Chalcone Synthase--F...)
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[[Image:1jwx.jpg|left|200px]]<br /><applet load="1jwx" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jwx, resolution 1.63&Aring;" />
 
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'''Chalcone Synthase--F215S mutant'''<br />
 
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==Overview==
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==Chalcone Synthase--F215S mutant==
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Type III polyketide synthases (PKS) generate an array of natural products, by condensing multiple acetyl units derived from malonyl-CoA to, thioester-linked starter molecules covalently bound in the PKS active, site. One strategy adopted by Nature for increasing the functional, diversity of these biosynthetic enzymes involves modifying polyketide, assembly by altering the preference for starter molecules. Chalcone, synthase (CHS) is a ubiquitous plant PKS and the first type III PKS, described functionally and structurally. Guided by the three-dimensional, structure of CHS, Phe-215 and Phe-265, which are situated at the active, site entrance, were targeted for site-directed mutagenesis to diversify, CHS activity. The resulting mutants were screened against a panel of, aliphatic and aromatic CoA-linked starter molecules to evaluate the degree, of starter molecule specificity in CHS. Although wild-type CHS accepts a, number of natural CoA thioesters, it does not use N-methylanthraniloyl-CoA, as a substrate. Substitution of Phe-215 by serine yields a CHS mutant that, preferentially accepts this CoA-thioester substrate to generate a novel, alkaloid, namely N-methylanthraniloyltriacetic acid lactone. These results, demonstrate that a point mutation in CHS dramatically shifts the molecular, selectivity of this enzyme. This structure-based approach to metabolic, redesign represents an initial step toward tailoring the biosynthetic, activity of plant type III PKS.
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<StructureSection load='1jwx' size='340' side='right'caption='[[1jwx]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1jwx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JWX FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.63&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jwx OCA], [https://pdbe.org/1jwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jwx RCSB], [https://www.ebi.ac.uk/pdbsum/1jwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jwx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CHS2_MEDSA CHS2_MEDSA] The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.<ref>PMID:10653632</ref> <ref>PMID:11732902</ref> <ref>PMID:11959984</ref> <ref>PMID:15380179</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jw/1jwx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jwx ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1JWX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Medicago_sativa Medicago sativa]. Active as [http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JWX OCA].
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*[[Chalcone synthase|Chalcone synthase]]
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== References ==
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==Reference==
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<references/>
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Expanding the biosynthetic repertoire of plant type III polyketide synthases by altering starter molecule specificity., Jez JM, Bowman ME, Noel JP, Proc Natl Acad Sci U S A. 2002 Apr 16;99(8):5319-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11959984 11959984]
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Medicago sativa]]
[[Category: Medicago sativa]]
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[[Category: Naringenin-chalcone synthase]]
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[[Category: Bowman ME]]
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[[Category: Single protein]]
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[[Category: Jez JM]]
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[[Category: Bowman, M.E.]]
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[[Category: Noel JP]]
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[[Category: Jez, J.M.]]
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[[Category: Noel, J.P.]]
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[[Category: altered substrate specificity]]
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[[Category: ketoacyl synthase]]
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[[Category: polyketide synthase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:38:55 2007''
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Current revision

Chalcone Synthase--F215S mutant

PDB ID 1jwx

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