2np0

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{{Seed}}
 
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[[Image:2np0.png|left|200px]]
 
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==Crystal structure of the Botulinum neurotoxin type B complexed with synaptotagamin-II ectodomain==
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The line below this paragraph, containing "STRUCTURE_2np0", creates the "Structure Box" on the page.
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<StructureSection load='2np0' size='340' side='right'caption='[[2np0]], [[Resolution|resolution]] 2.62&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2np0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NP0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NP0 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.62&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2np0| PDB=2np0 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2np0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2np0 OCA], [https://pdbe.org/2np0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2np0 RCSB], [https://www.ebi.ac.uk/pdbsum/2np0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2np0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/BXB_CLOBO BXB_CLOBO] Botulinum toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that cleaves the '76-Gln-|-Phe-77' bond of synaptobrevin-2.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/np/2np0_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2np0 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Botulinum neurotoxins (BoNTs) are potent bacterial toxins that cause paralysis at femtomolar concentrations by blocking neurotransmitter release. A 'double receptor' model has been proposed in which BoNTs recognize nerve terminals via interactions with both gangliosides and protein receptors that mediate their entry. Of seven BoNTs (subtypes A-G), the putative receptors for BoNT/A, BoNT/B and BoNT/G have been identified, but the molecular details that govern recognition remain undefined. Here we report the crystal structure of full-length BoNT/B in complex with the synaptotagmin II (Syt-II) recognition domain at 2.6 A resolution. The structure of the complex reveals that Syt-II forms a short helix that binds to a hydrophobic groove within the binding domain of BoNT/B. In addition, mutagenesis of amino acid residues within this interface on Syt-II affects binding of BoNT/B. Structural and sequence analysis reveals that this hydrophobic groove is conserved in the BoNT/G and BoNT/B subtypes, but varies in other clostridial neurotoxins. Furthermore, molecular docking studies using the ganglioside G(T1b) indicate that its binding site is more extensive than previously proposed and might form contacts with both BoNT/B and synaptotagmin. The results provide structural insights into how BoNTs recognize protein receptors and reveal a promising target for blocking toxin-receptor recognition.
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===Crystal structure of the Botulinum neurotoxin type B complexed with synaptotagamin-II ectodomain===
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Structural basis of cell surface receptor recognition by botulinum neurotoxin B.,Chai Q, Arndt JW, Dong M, Tepp WH, Johnson EA, Chapman ER, Stevens RC Nature. 2006 Dec 21;444(7122):1096-100. Epub 2006 Dec 13. PMID:17167418<ref>PMID:17167418</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2np0" style="background-color:#fffaf0;"></div>
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==See Also==
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The line below this paragraph, {{ABSTRACT_PUBMED_17167418}}, adds the Publication Abstract to the page
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*[[Botulinum neurotoxin 3D structures|Botulinum neurotoxin 3D structures]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17167418 is the PubMed ID number.
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*[[Synaptotagmin 3D structures|Synaptotagmin 3D structures]]
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== References ==
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{{ABSTRACT_PUBMED_17167418}}
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<references/>
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__TOC__
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==About this Structure==
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</StructureSection>
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2NP0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Clostridium_botulinum Clostridium botulinum] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NP0 OCA].
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==Reference==
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Structural basis of cell surface receptor recognition by botulinum neurotoxin B., Chai Q, Arndt JW, Dong M, Tepp WH, Johnson EA, Chapman ER, Stevens RC, Nature. 2006 Dec 21;444(7122):1096-100. Epub 2006 Dec 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17167418 17167418]
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[[Category: Bontoxilysin]]
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[[Category: Clostridium botulinum]]
[[Category: Clostridium botulinum]]
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[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
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[[Category: Protein complex]]
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[[Category: Arndt JW]]
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[[Category: Arndt, J W.]]
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[[Category: Chai Q]]
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[[Category: Chai, Q.]]
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[[Category: Stevens RC]]
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[[Category: Stevens, R C.]]
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[[Category: Botulinum]]
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[[Category: Neurotoxin]]
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[[Category: Receptor]]
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[[Category: Synaptotagamin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:00:43 2008''
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Current revision

Crystal structure of the Botulinum neurotoxin type B complexed with synaptotagamin-II ectodomain

PDB ID 2np0

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