2yvz

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{{Seed}}
 
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[[Image:2yvz.png|left|200px]]
 
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==Crystal structure of magnesium transporter MgtE cytosolic domain, Mg2+-free form==
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The line below this paragraph, containing "STRUCTURE_2yvz", creates the "Structure Box" on the page.
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<StructureSection load='2yvz' size='340' side='right'caption='[[2yvz]], [[Resolution|resolution]] 3.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2yvz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Thet8 Thet8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YVZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=2YVZ FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yvx|2yvx]], [[2yvy|2yvy]]</td></tr>
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{{STRUCTURE_2yvz| PDB=2yvz | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=2yvz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yvz OCA], [http://pdbe.org/2yvz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2yvz RCSB], [http://www.ebi.ac.uk/pdbsum/2yvz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2yvz ProSAT]</span></td></tr>
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</table>
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== Function ==
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[[http://www.uniprot.org/uniprot/MGTE_THET8 MGTE_THET8]] Acts as a highly selective magnesium channel.<ref>PMID:17700703</ref> <ref>PMID:19798051</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yv/2yvz_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2yvz ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The magnesium ion Mg2+ is a vital element involved in numerous physiological processes. Mg2+ has the largest hydrated radius among all cations, whereas its ionic radius is the smallest. It remains obscure how Mg2+ transporters selectively recognize and dehydrate the large, fully hydrated Mg2+ cation for transport. Recently the crystal structures of the CorA Mg2+ transporter were reported. The MgtE family of Mg2+ transporters is ubiquitously distributed in all phylogenetic domains, and human homologues have been functionally characterized and suggested to be involved in magnesium homeostasis. However, the MgtE transporters have not been thoroughly characterized. Here we determine the crystal structures of the full-length Thermus thermophilus MgtE at 3.5 A resolution, and of the cytosolic domain in the presence and absence of Mg2+ at 2.3 A and 3.9 A resolutions, respectively. The transporter adopts a homodimeric architecture, consisting of the carboxy-terminal five transmembrane domains and the amino-terminal cytosolic domains, which are composed of the superhelical N domain and tandemly repeated cystathionine-beta-synthase domains. A solvent-accessible pore nearly traverses the transmembrane domains, with one potential Mg2+ bound to the conserved Asp 432 within the pore. The transmembrane (TM)5 helices from both subunits close the pore through interactions with the 'connecting helices', which connect the cystathionine-beta-synthase and transmembrane domains. Four putative Mg2+ ions are bound at the interface between the connecting helices and the other domains, and this may lock the closed conformation of the pore. A structural comparison of the two states of the cytosolic domains showed the Mg2+-dependent movement of the connecting helices, which might reorganize the transmembrane helices to open the pore. These findings suggest a homeostasis mechanism, in which Mg2+ bound between cytosolic domains regulates Mg2+ flux by sensing the intracellular Mg2+ concentration. Whether this presumed regulation controls gating of an ion channel or opening of a secondary active transporter remains to be determined.
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===Crystal structure of magnesium transporter MgtE cytosolic domain, Mg2+-free form===
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Crystal structure of the MgtE Mg2+ transporter.,Hattori M, Tanaka Y, Fukai S, Ishitani R, Nureki O Nature. 2007 Aug 30;448(7157):1072-5. Epub 2007 Aug 15. PMID:17700703<ref>PMID:17700703</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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The line below this paragraph, {{ABSTRACT_PUBMED_17700703}}, adds the Publication Abstract to the page
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<div class="pdbe-citations 2yvz" style="background-color:#fffaf0;"></div>
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(as it appears on PubMed at http://www.pubmed.gov), where 17700703 is the PubMed ID number.
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== References ==
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<references/>
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{{ABSTRACT_PUBMED_17700703}}
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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2YVZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YVZ OCA].
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[[Category: Thet8]]
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[[Category: Fukai, S]]
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==Reference==
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[[Category: Hattori, M]]
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Crystal structure of the MgtE Mg2+ transporter., Hattori M, Tanaka Y, Fukai S, Ishitani R, Nureki O, Nature. 2007 Aug 30;448(7157):1072-5. Epub 2007 Aug 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17700703 17700703]
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[[Category: Ishitani, R]]
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[[Category: Single protein]]
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[[Category: Nureki, O]]
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[[Category: Thermus thermophilus]]
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[[Category: Tanaka, Y]]
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[[Category: Fukai, S.]]
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[[Category: Hattori, M.]]
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[[Category: Ishitani, R.]]
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[[Category: Nureki, O.]]
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[[Category: Tanaka, Y.]]
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[[Category: Membrane protein]]
[[Category: Membrane protein]]
[[Category: Transport protein]]
[[Category: Transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:01:54 2008''
 

Current revision

Crystal structure of magnesium transporter MgtE cytosolic domain, Mg2+-free form

PDB ID 2yvz

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