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1jxh

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(New page: 200px<br /><applet load="1jxh" size="450" color="white" frame="true" align="right" spinBox="true" caption="1jxh, resolution 2.3&Aring;" /> '''4-Amino-5-hydroxymeth...)
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[[Image:1jxh.gif|left|200px]]<br /><applet load="1jxh" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1jxh, resolution 2.3&Aring;" />
 
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'''4-Amino-5-hydroxymethyl-2-methylpyrimidine Phosphate Kinase from Salmonella typhimurium'''<br />
 
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==Overview==
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==4-Amino-5-hydroxymethyl-2-methylpyrimidine Phosphate Kinase from Salmonella typhimurium==
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The crystal structures of Salmonella typhimurium, 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase (HMPP kinase), and its complex with substrate HMP have been determined. HMPP kinase, catalyzes two separate ATP-dependent phosphorylation reactions and is an, essential enzyme in the thiamin biosynthetic pathway. HMPP kinase is a, homodimer with one active site per monomer and is structurally homologous, to members of the ribokinase family. A comparison of the structure of HMPP, kinase with other members of the ribokinase family suggests an, evolutionary progression. Modeling studies suggest that HMPP kinase, catalyzes both of its phosphorylation reactions using in-line displacement, mechanisms. We propose that the active site accommodates the two separate, reactions by providing two different binding modes for the phosphate group, of HMP phosphate.
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<StructureSection load='1jxh' size='340' side='right'caption='[[1jxh]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1jxh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JXH FirstGlance]. <br>
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1JXH is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phosphomethylpyrimidine_kinase Phosphomethylpyrimidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.4.7 2.7.4.7] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1JXH OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jxh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jxh OCA], [https://pdbe.org/1jxh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jxh RCSB], [https://www.ebi.ac.uk/pdbsum/1jxh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jxh ProSAT]</span></td></tr>
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Crystal structure of 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase from Salmonella typhimurium at 2.3 A resolution., Cheng G, Bennett EM, Begley TP, Ealick SE, Structure. 2002 Feb;10(2):225-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11839308 11839308]
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</table>
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[[Category: Phosphomethylpyrimidine kinase]]
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== Function ==
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[[Category: Salmonella typhimurium]]
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[https://www.uniprot.org/uniprot/THID_SALTY THID_SALTY] Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P (By similarity).
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[[Category: Single protein]]
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== Evolutionary Conservation ==
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[[Category: Begley, T.P.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Bennett, E.M.]]
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Check<jmol>
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[[Category: Cheng, G.]]
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<jmolCheckbox>
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[[Category: Ealick, S.E.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/jx/1jxh_consurf.spt"</scriptWhenChecked>
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[[Category: SO4]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: kinase]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: phophorylation]]
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</jmolCheckbox>
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[[Category: ribokinase family]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1jxh ConSurf].
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[[Category: thid]]
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<div style="clear:both"></div>
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__TOC__
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 18:39:56 2007''
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Begley TP]]
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[[Category: Bennett EM]]
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[[Category: Cheng G]]
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[[Category: Ealick SE]]

Current revision

4-Amino-5-hydroxymethyl-2-methylpyrimidine Phosphate Kinase from Salmonella typhimurium

PDB ID 1jxh

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