2mlp

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:57, 23 October 2024) (edit) (undo)
 
(10 intermediate revisions not shown.)
Line 1: Line 1:
-
{{Seed}}
 
-
[[Image:2mlp.png|left|200px]]
 
-
<!--
+
==MICROCIN LEADER PEPTIDE FROM E. COLI, NMR, 25 STRUCTURES==
-
The line below this paragraph, containing "STRUCTURE_2mlp", creates the "Structure Box" on the page.
+
<StructureSection load='2mlp' size='340' side='right'caption='[[2mlp]]' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[2mlp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MLP FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 25 models</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-
{{STRUCTURE_2mlp| PDB=2mlp | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mlp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mlp OCA], [https://pdbe.org/2mlp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mlp RCSB], [https://www.ebi.ac.uk/pdbsum/2mlp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mlp ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/MCBA_ECOLX MCBA_ECOLX] This glycine-rich peptide antibiotic inhibits DNA replication in many enteric bacteria, that leads to induction of the SOS repair system, massive DNA degradation and cell death. B17 inhibits type II topoisomerase by trapping an enzyme - DNA cleavable complex.<ref>PMID:1846808</ref>
 +
<div style="background-color:#fffaf0;">
 +
== Publication Abstract from PubMed ==
 +
BACKGROUND: The peptide antibiotic microcin B17 (MccB17) contains oxazole and thiazole heterocycles formed by the post-translational modification of four cysteine and four serine residues. An amino-terminal propeptide targets the 69 amino acid precursor of MccB17 (preproMccB17) to the heterocyclization enzyme MccB17 synthetase. The mode of synthetase recognition has been unclear, because there has been limited structural information available on the MccB17 propeptide to date. RESULTS: The solution structure of the MccB17 propeptide (McbA1-26), determined using nuclear magnetic resonance, reveals that McbA1-26 is an amphipathic alpha helix. Mutational analysis of 13 propeptide residues showed that Phe8 and Leu12 are essential residues for MccB17 synthetase recognition. A domain of the propeptide was putatively identified as the region that interacts with the synthetase. CONCLUSIONS: MccB17 synthetase recognizes key hydrophobic residues within a helical propeptide, allowing the selective heterocyclization of downstream cysteine and serine residues in preproMccB17. The determination of the solution structure of the propeptide should facilitate the investigation of other functions of the propeptide, including a potential role in antibiotic secretion.
-
===MICROCIN LEADER PEPTIDE FROM E. COLI, NMR, 25 STRUCTURES===
+
Role of the microcin B17 propeptide in substrate recognition: solution structure and mutational analysis of McbA1-26.,Roy RS, Kim S, Baleja JD, Walsh CT Chem Biol. 1998 Apr;5(4):217-28. PMID:9545435<ref>PMID:9545435</ref>
-
 
+
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-
<!--
+
</div>
-
The line below this paragraph, {{ABSTRACT_PUBMED_9545435}}, adds the Publication Abstract to the page
+
<div class="pdbe-citations 2mlp" style="background-color:#fffaf0;"></div>
-
(as it appears on PubMed at http://www.pubmed.gov), where 9545435 is the PubMed ID number.
+
== References ==
-
-->
+
<references/>
-
{{ABSTRACT_PUBMED_9545435}}
+
__TOC__
-
 
+
</StructureSection>
-
==About this Structure==
+
-
2MLP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MLP OCA].
+
-
 
+
-
==Reference==
+
-
Role of the microcin B17 propeptide in substrate recognition: solution structure and mutational analysis of McbA1-26., Roy RS, Kim S, Baleja JD, Walsh CT, Chem Biol. 1998 Apr;5(4):217-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9545435 9545435]
+
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Baleja, J D.]]
+
[[Category: Baleja JD]]
-
[[Category: Kim, S.]]
+
[[Category: Kim S]]
-
[[Category: Roy, R Sinha.]]
+
[[Category: Sinha Roy R]]
-
[[Category: Walsh, C T.]]
+
[[Category: Walsh CT]]
-
[[Category: Antibiotic]]
+
-
[[Category: Leader peptide]]
+
-
[[Category: Propeptide]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:05:05 2008''
+

Current revision

MICROCIN LEADER PEPTIDE FROM E. COLI, NMR, 25 STRUCTURES

PDB ID 2mlp

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mlp"
Personal tools